响应面法优化蛋壳膜中硫酸软骨素提取工艺  

Optimization of Chondroitin Sulfate Extraction from Eggshell Membrane by Response Surface Method

作　　者：刘恒洋 尹玉鑫 郭晓徐 宋绍奇 刘美玉 LIU Hengyang;YIN Yuxin;GUO Xiaoxu;SONG Shaoqi;LIU Meiyu(College of Life Science and Food Engineering,Hebei University of Engineering,Handan,Hebei 056038,China;Handan Key Laboratory of Natural Products and Functional Food Development,Handan,Hebei 056038,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,河北邯郸056038 [2]邯郸市天然产物与功能性食品开发重点室验室,河北邯郸056038

出　　处：《农产品加工》2023年第23期46-50,53,共6页Farm Products Processing

基　　金：河北省重点研发计划项目-农业高质量发展关键共性技术攻关专项项目(19227127D);邯郸市科技研究与发展计划项目(21422012249)。

摘　　要：以蛋壳膜为原材料,利用碱盐法从中进行硫酸软骨素的提取试验,以硫酸软骨素的提取率为指标,探究不同提取时间、提取温度、碱盐比及料液比对硫酸软骨素提取率的影响。在单因素试验的基础上设计相应面试验对提取工艺进行优化。结果表明,蛋壳膜为1 g,最佳工艺为料液比为15.5∶1,提取温度为60℃,碱盐比为6∶1,提取时间为8 h。在此条件下提取率可达76.5%。Eggshell membrane as raw materials,the use of alkali salt method from the chondroitin sulfate extraction test,chondroitin sulfate extraction rate as an index,explored different extraction time,extraction temperature,alkali salt ratio,and solid-liquid ratio of chondroitin sulfate extraction rate.On the basis of single factor,the corresponding surface experiment was designed to optimize the extraction process.The results showed that the eggshell membrane was 1 g,the optimum technology was solid-liquid ratio 15.5∶1,extraction temperature 60℃,alkali-salt ratio 6∶1,and extraction time 8 h.Under these conditions,the extraction yield could reached 76.5%.

关 键 词：蛋壳膜 硫酸软骨素 碱盐法 响应面法 

分 类 号：TS253.9[轻工技术与工程—农产品加工及贮藏工程]