基于粪便DNA的四川省色达县野生食肉动物棘球绦虫感染调查  

Prevalence of Echinococcus infections in wild carnivores based on copro⁃DNA tests in Serthar County of Sichuan Province

作　　者：杨柳 杨毅 喻文杰[1] 王谦[1] 钟波[1] 花开琼 刘阳[1] 黄燕[1] YANG Liu;YANG Yi;YU Wenjie;WANG Qian;ZHONG Bo;HUA Kaiqiong;LIU Yang;HUANG Yan(Sichuan Provincial Center for Disease Control and Prevention,Chengdu,Sichuang 610041,China;Serthar County Center for Disease Control and Prevention,Garze Tibetan Autonomous Prefecture,Sichuan Province,China)

机构地区：[1]四川省疾病预防控制中心,四川成都610041 [2]四川省色达县疾病预防控制中心

出　　处：《中国血吸虫病防治杂志》2023年第5期492-496,共5页Chinese Journal of Schistosomiasis Control

基　　金：四川省科技计划项目(2023YFS0221)。

摘　　要：目的了解四川省色达县野生食肉动物棘球绦虫感染率,为当地开展棘球蚴病防控工作提供参考依据。方法于2021年5月在四川省色达县采集野生食肉动物粪便样本,采用PCR法鉴定粪便样本的来源宿主及棘球绦虫感染情况。分别计算不同宿主多房、细粒、石渠棘球绦虫感染率,对其差异进行统计学比较。结果共采集野生食肉动物粪便样本583份,其中犬科动物粪便样本528份,宿主来源为狐狸、狼、野犬的粪便样本分别为147、154、227份;猫科动物粪便样本11份,宿主来源均为猞猁。野生犬科动物粪便样本多房、细粒、石渠棘球绦虫总感染率分别为5.68%(30/528)、0.19%(1/528)、14.20%(75/528)。狐狸粪便样本未检出细粒棘球绦虫感染,多房、石渠棘球绦虫感染率分别为0.68%(1/147)、47.62%(70/147),差异有统计学意义(χ^(2)=88.41,P<0.001);狼粪便样本未检出细粒、石渠棘球绦虫感染,多房棘球绦虫感染率为10.39%(16/154);野犬粪便样本多房、细粒、石渠棘球绦虫感染率分别为5.73%(13/227)、0.44%(1/227)、2.20%(5/227),不同虫种棘球绦虫感染率差异有统计学意义(χ^(2)=12.13,P<0.01)。狼粪便样本多房棘球绦虫感染率显著高于野犬、狐狸(χ^(2)=13.23,P<0.01)。狐狸粪便样本的石渠棘球绦虫感染率显著高于野犬、狼(χ^(2)=187.01,P<0.001)。11份猞猁粪便样本中,未见棘球绦虫感染。结论四川省色达县野生犬科动物棘球绦虫感染率较高,狼、野犬、狐狸均参与色达县多房棘球绦虫生活史的野外循环,其中狼、野犬或发挥更为重要的作用。Objective To investigate the prevalence of Echinococcus infections in wild carnivores in Serthar County,Sichuan Province,so as to provide insights into echinococcosis control in local areas.Methods Stool samples were collected from wild carnivores in Serthar County,Sichuan Province in May 2021,and the host sources of stool samples and Echinococcus infections were identified using PCR assays.The prevalence of E.multilocularis,E.granulosus and E.shiquicus infections was estimated in different hosts.Results A total of 583 stool samples were collected from wild carnivores,including 147 stool samples from fox,154 from wolf,227 from wild dogs and 11 from lynx.The overall prevalence of E.multilocularis,E.granulosus and E.shiquicus infections was 5.68%,0.19%and 14.20%in canine stool samples,and no E.granulosus infection was detected in fox stool samples,while the prevalence of E.multilocularis and E.shiquicus infections was 0.68%and 47.62%in fox stool samples([χ^(2)]=88.41,P<0.001).No E.granulosus or E.shiquicus infection was detected in wolf stool samples,and the prevalence of E.multilocularis infection was 10.39%in wolf stool samples.The prevalence of E.multilocularis,E.granulosus and E.shiquicus infections was 5.73%,0.44%and 2.20%in canine stool samples([χ^(2)]=12.13,P<0.01).In addition,the prevalence of E.multilocularis infections was significantly higher in wolf stool samples than in canine and fox stool samples([χ^(2)]=13.23,P<0.01),and the prevalence of E.shiquicus infections was significantly higher in fox stool samples than in canine and wolf stool samples([χ^(2)]=187.01,P<0.001).No Echinococcus infection was identified in 11 lynx stool samples.Conclusions The prevalence of Echinococcus infections is high in wild canines in Serthar County,Sichuan Province.Wolf,wild dog and fox all participate in the wild life cycle of E.multilocularis in Serthar County,and wolf and wild dogs may play a more important role.

关 键 词：棘球绦虫 野生食肉动物 粪便DNA 色达县 

分 类 号：R383.33[医药卫生—医学寄生虫学]