芍药苷对舌鳞癌CAL27细胞的抑制作用及机制探讨  

Inhibitory effect of paeoniflorin on CAL27 cells of tongue squamous cell carcinoma and its mechanism

作　　者：刘芸 陈浩月 米静 王菲菲[3] 万光勇 崔超[4] LIU Yun;CHEN Hao-yue;MI Jing;WANG Fei-fei;WAN Guang-yong;CUI Chao(School of Stomatology,Weifang Medical University,Weifang 261053;Department of Rehabilitation,The Affiliated Taian City Central Hospital of Qingdao University,Taian 271000,Shandong Province,China;Department of Stomatology,The Affiliated Taian City Central Hospital of Qingdao University,Taian 271000,Shandong Province,China;Hydrogen Medical Research Center,The Affiliated Taian City Central Hospital of Qingdao University,Taian 271000,Shandong Province,China)

机构地区：[1]潍坊医学院口腔医学院,山东潍坊261053 [2]青岛大学附属泰安市中心医院康复科,山东泰安271000 [3]青岛大学附属泰安市中心医院口腔科,山东泰安271000 [4]青岛大学附属泰安市中心医院氢医学研究中心,山东泰安271000

出　　处：《上海口腔医学》2023年第6期597-602,共6页Shanghai Journal of Stomatology

摘　　要：目的:探讨不同浓度芍药苷(paeoniflorin,PF)对舌鳞癌CAL27细胞的体外抑制作用及可能的作用机制。方法:通过CCK-8法及克隆形成实验,研究PF对CAL27细胞增殖能力、克隆形成能力的影响。采用划痕实验及Transwell法,检测PF对CAL27细胞迁移和侵袭能力的影响。采用Hoechst33342染色,观察PF对CAL27细胞凋亡的影响。采用蛋白质免疫印迹(Western blot),研究PF对NF-κB通路及EMT相关蛋白表达的影响。通过一氧化氮检测试剂盒,检测PF对CAL27细胞NO产生量的影响。采用SPSS 27.0软件包对数据进行统计学分析。结果:PF在体外条件下对CAL27细胞的增殖、迁移及侵袭有抑制作用,且其抑制效应呈浓度依赖性。此外,PF促进CAL27细胞发生凋亡。PF可抑制NF-κB通路活化,降低P-P65表达,进而降低i NOS及MMP-9表达,抑制NO生成;同时抑制Vimentin表达,促进E-cadherin表达,最终抑制上皮-间质转化(EMT)。结论:PF抑制CAL27细胞增殖、迁移及侵袭,可能通过抑制NF-κB通路活化、抑制EMT而发挥抗癌作用。PURPOSE:To investigate the inhibitory effect of paeoniflorin(PF)with different concentrations on CAL27 cells of tongue squamous cell carcinoma in vitro and its possible mechanism.METHODS:CCK-8 technique and clone formation trial were used to detect the effect of PF on the proliferation and clone formation of CAL27 cells.Scratch test and Transwell method was used to detect the effects of PF on migration and invasion of CAL27 cells.Staining of Hoechst33342 was employed to evaluate the influence of PF on apoptosis of CAL27 cells,while Western blot was utilized to investigate the effect of PF on the expression of NF-κB pathway related proteins and EMT related proteins.The effect of PF on NO production in CAL27 cells was detected by nitric oxide detection kit.Statistical analysis was performed with SPSS 27.0 software package.RESULTS:PF inhibited proliferation,migration,and invasion of CAL27 cells in vitro in a concentration-dependent way.Moreover,PF caused apoptosis of CAL27 cells.PF impeded NF-κB pathway activity,decreased the expression of P-P65,further reduced the expression of iNOS and MMP-9,suppressed the production of NO,and concurrently inhibited Vimentin,promoted E-cadherin.CONCLUSIONS:Paeoniflorin inhibits the proliferation,migration and invasion of CAL27 cells,which may play an anti-cancer role by inhibiting the activation of NF-κB pathway and EMT.

关 键 词：芍药苷 CAL27细胞 NF-ΚB 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]