NEK2与EMT相关分子在口腔鳞状细胞癌中的表达及临床意义  

Expression and clinical significance of NEK2 and EMT related molecules in oral squamous cell carcinoma

作　　者：叶婷婷 陈蔚华[1] 裴婧[1] 贾云香[1] 吴海莹 YE Ting-ting;CHEN Wei-hua;PEI Jing;JIA Yun-xiang;WU Hai-ying(Department of Pathology,Affiliated Stomatological Hospital of Nanchang University/Key Laboratory of Oral Biomedicine,Jiangxi Province/Jiangxi Province Clinical Research Center for Oral Diseases,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]南昌大学附属口腔医院病理科,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西南昌330006

出　　处：《上海口腔医学》2023年第6期640-644,共5页Shanghai Journal of Stomatology

基　　金：江西省重点研发计划一般项目(S2020ZPYFB2025)。

摘　　要：目的:探讨NEK2与EMT相关分子在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及意义。方法:采用免疫组织化学方法检测60例原发性OSCC组织及正常口腔黏膜组织中NEK2和EMT相关分子(E-Cadherin、N-Cadherin、Vimentin)的表达水平,采用实时荧光定量PCR(qRT-PCR)检测25例OSCC组织和10例正常口腔黏膜中NEK2 mRNA的表达水平。采用SPSS 26.0软件包对数据进行统计学分析。结果:OSCC组织中NEK2、NCadherin、Vimentin表达显著升高,E-Cadherin表达显著下降(P<0.05);分化越差,NEK2表达越高,E-Cadherin表达越低(P<0.05)。结论:NEK2可作为初步判断OSCC预后的标志物。NEK2的表达上调,EMT相关分子表达水平的变化导致细胞间黏附力下降,侵袭性增强,促进肿瘤的发生、发展。PURPOSE:To investigate the expression and significance of NEK2 and EMT-related molecules in oral squamous cell carcinoma(OSCC).METHODS:The expression levels of NEK2 and EMT-related molecules(E-Cadherin,N-Cadherin,Vimentin)in 60 cases of primary OSCC tissues and normal oral mucosa tissues were detected by immunohistochemistry(IHC).NEK2 mRNA expression in 25 OSCC tissues and 10 normal oral mucosa tissues was detected by qRT-PCR.Statistical analysis was performed using SPSS 26.0 software package.RESULTS:The expression of NEK2,N-Cadherin,and Vimentin increased in OSCC tissues,while the expression of E-Cadherin decreased(P<0.05).The worse the differentiation,the higher the expression of NEK2 and the lower the expression of E-Cadherin(P<0.05).CONCLUSIONS:NEK2 can be used as a prognostic marker for OSCC.The up-regulation of NEK2 and the changes in the expression levels of EMT related molecules can promote the occurrence and development of OSCC.

关 键 词：口腔鳞状细胞癌 NEK2 EMT 预后 

分 类 号：R739.8[医药卫生—肿瘤]