不同微载体细胞培养技术在生物制品领域的应用  

Application of different microcarrier cell culture technologies in biological product field

作　　者：王晓勋(综述) 张中洋(审校) WANG Xiaoxun;ZHANG Zhongyang(National Vaccine and Serum Institute,Beijing 1011ll,China)

机构地区：[1]国药中生生物技术研究院有限公司,北京101111

出　　处：《中国生物制品学杂志》2023年第12期1515-1521,1529,共8页Chinese Journal of Biologicals

基　　金：国家卫生计生委专项课题(2018ZX09737011)。

摘　　要：单位面积细胞的生产力决定了细胞培养工艺放大潜力,微载体系统的规模化应用可为贴壁依赖型动物细胞的高产培养提供极大的表面积,适合高效生产工艺的开发和优化放大。近年,基于微载体的培养工艺广泛用于多种类型动物细胞培养,可生产多种重要的生物制品,如疫苗、酶类、激素、抗体、干扰素及其他生物制剂等。本文就国内外微载体细胞培养技术的研究进展、新型微载体和传统微载体的比较及其应用作一综述,以期为新型微载体大规模细胞培养技术的深入研究及应用提供参考。The productivity of cells per unit area determines the scale-up potential of the cell culture process,and the largescale application of the microcarrier system provides space for the high-yield culture of anchorage-dependent animal cells.The microcarrier animal cell culture technology is suitable for efficient production process development and optimized amplification.In recent years,microcarrier-based culture technology has been widely used in various types of animal cell culture to produce many important biological products such as vaccines,enzymes,hormones,antibodies,interferons and other probiotics.In this paper,the research progress of domestic and foreign microcarrier cell culture technology,the comparison of new microcarriers and traditional microcarriers and their applications were reviewed,so as to provide reference for the in-depth research and application of large-scale cell culture technology based on new microcarriers.

关 键 词：微载体 细胞培养 生物制品 

分 类 号：Q813.1[生物学—生物工程]