甘菊ClKRPs转录因子的鉴定及分子生物学分析  

Identification and Molecular Biology Analysis of ClKRPs Transcription Factor in Chrysanthemum lavandulifolium

作　　者：杨雯婷 张佳凝 何子涵 任镘蓉 全英杰 高日[1] 赵丽 Yang Wenting;Zhang Jianing;He Zihan;Ren Manrong;Quan Yingjie;Gao Ri;Zhao Li(Agricultural College of Yanbian University,Yanji,133400;Yanbian Academy of Forestry Sciences,Yanji,133002)

机构地区：[1]延边大学农学院,延吉133400 [2]延边林业科学研究院,延吉133002

出　　处：《分子植物育种》2024年第1期1-7,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(32060696);延边州科技发展计划项目(2020FG31);延边大学校企合作项目(延大科合字2020);延边大学青年基金项目(延大科合字[2017]第21号);延边大学博士科研启动基金项目(2018);农业农村部景观设计重点实验室开放课题(KF201902)共同资助。

摘　　要：甘菊[Chrysanthemum lavandulifolium(Fisch.ex Trautv.) Ling et Shih]为菊科菊属植物,二倍体,基因组较小,为栽培菊花的亲本之一。KRP是植物中独特的转录因子,参与细胞周期调控,影响植物生长和发育。本试验以甘菊为材料,克隆ClKRPs基因,分析其基本理化性质、构建系统进化树、miRNA靶基因预测、保守基序分析。结果表明,ClKRPs转录因子相对分子量为29.10~144.30,ClKRPs蛋白都为酸性蛋白;ClKRP1、ClKRP2、ClKRP3、ClKRP5、ClKRP6、ClKRP10、ClKRP12、ClKRP14、ClKRP15蛋白为稳定蛋白,ClKRP4、ClKRP7、ClKRP8、ClKRP9、ClKRP11、ClKRP13、ClKRP16为不稳定蛋白。系统发育分析显示,甘菊ClKRPs蛋白可以分为两大类。利用MEME软件识别出6个甘菊ClKRP保守基序。生物信息学分析预测5个ClKRP基因与4个miRNA结合。本研究为进一步探索和分析甘菊ClKRPs转录因子的功能奠定了基础。Chamomile [Chrysanthemum lavandulifolium(Fisch.ex Trautv.) Ling et Shih] is a plant of Chrysanthemum genus in the family of Asteraceae,which is diploid and has a small genome.It is one of the parents of cultivated chrysanthemums.KRP is a unique type of transcription factor in plants,which participates in cell cycle regulation and affects plant growth and development.In this experiment,chamomile was used as the material to clone the ClKRPs gene,analyze its basic physical and chemical properties,construct a phylogenetic tree,predict miRNA target genes,and analyze conservative motifs.The results showed that:ClKRPs transcription factor relative molecular weight was 29.10~144.30,ClKRPs proteins were acidic proteins;ClKRP1,ClKRP2,ClKRP3,ClKRP5,ClKRP6,ClKRP10,ClKRP12,ClKRP14,ClKRP15 proteins were stable proteins,ClKRP4,ClKRP7,ClKRP8,ClKRP9,ClKRP11,ClKRP13,ClKRP16 were unstable proteins.Phylogenetic analysis showed that the ClKRPs protein of chamomile could be divided into two categories.Six conserved motifs of camomile ClKRP were identified by MEME software.Bioinformatics analysis predicts that 5 ClKRP genes bound to 4 miRNAs.This study laid the foundation for further mining and analysis of the functions of the chamomile ClKRPs transcription factors.

关 键 词：甘菊(Chrysanthemum lavandulifolium) ClKRPs 系统进化树 理化性质 

分 类 号：S682.11[农业科学—观赏园艺]