微生物实验教学中氧化酶试验准确性和稳定性的探讨  

Evaluation of the Accuracy and Stability of Oxidase Test in Microbiology Experimental Teaching Course

作　　者：权云帆 曾颖斐 李金谦 符婷婷 孙庆惠 修皓 崔秀吉 QUAN Yunfan;ZENG Yingfei;LI Jinqian;FU Tingting;SUN Qinghui;XIU Hao;CUI Xiuji(School of Tropical Medicine,Hainan Medical University,Haikou 571199,China;School of Basic Medical Sciences and Life Sciences,Hainan Medical University,Haikou 571199,China)

机构地区：[1]海南医学院热带医学院,海口571199 [2]海南医学院基础医学与生命科学学院,海口571199

出　　处：《实验科学与技术》2023年第6期122-126,共5页Experiment Science and Technology

基　　金：海南省高等学校教育教学改革研究项目(Hnjg2021-67);海南医学院教育科研项目(HYYB202331)。

摘　　要：为提高实验教学操作中氧化酶试验的准确性和稳定性,减少和避免学生在操作中出现假阳性或假阴性的情况,尝试采用优化实验方法和铜绿假单胞菌培养基的手段。通过测试多种培养基和多种氧化酶试纸(试剂),选择稳定可靠的适用于学生实验的氧化酶试验方法。以灭菌竹签或玻璃棒取菌或直接用试纸蘸取菌落,取自普通营养琼脂斜面培养基上的铜绿假单胞菌氧化酶试验结果假阴性较多;取自普通营养琼脂平板或血琼脂平板培养基上的铜绿假单胞菌氧化酶试验结果均为阳性。实验结果表明,氧化酶试验以血琼脂平板培养基培养物效果最佳,采用氧化酶试纸或盐酸二甲基对苯二胺化学释放剂试纸均可。The study aims to increase the accuracy and stability of the oxidase test due to the frequent false-positive and falsenegative to the oxidase test during the microbiology experimental teaching.Here,we optimized experimental procedure and tested various culture mediums for pseudomonas aeruginosa by evaluating various bacterial culture medium and oxidase test kit to find a proper oxidase test protocol.Colonies were picked up with a sterilized toothpick,glass stick,or directly picked up with the paper disk.As a result,pseudomonas aeruginosa cultured in a slant agar medium frequently showed false positivity in the oxidase test,whereas pseudomonas aeruginosa cultured in regular agar plate or blood agar plate didn’t show any false positive.The experiment results showed that the blood agar was the best medium for bacterial culture for the oxidase test,and the oxidase test paper disk or N,NDimethyl-p-phenylenediamine dihydrochloride test paper showed a similar result in the oxidase test.

关 键 词：氧化酶试验 铜绿假单胞菌 血琼脂平板培养基 稳定性 

分 类 号：R-331[医药卫生]