一株耐甲醇的酯香微杆菌的特性研究  

Methanol-tolerant Strain of Microbacterium esteraromaticum and Its Properties

作　　者：张宇宁 鲁政武 王文博 高翠娟[1] 陈之群[1] 刘晶晶 ZHANG Yu-ning;LU Zheng-wu;WANG Wen-bo;GAO Cui-juan;CHEN Zhi-qun;LIU Jing-jing(Life science/Linyi University,Linyi 276000,China;Chemistry and Chemical Engineering/Linyi University,Linyi 276000,China)

机构地区：[1]临沂大学生命科学学院,山东临沂276000 [2]临沂大学化学化工学院,山东临沂276000

出　　处：《山东农业大学学报（自然科学版）》2023年第6期852-857,共6页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：山东省自然科学基金(ZR2020QC007);中央引导地方科技发展专项资金项目(YDZX2021070)。

摘　　要：本研究以自酿葡萄酒中分离出的一株酯香微杆菌(Microbacterium esteraromaticum)为研究对象,旨在探究该菌对不同浓度的甲醇和不同环境pH的耐受性、产絮凝剂的特性及其对不同油脂的降解能力。实验结果表明该菌可以在最高10%浓度的甲醇培养基中缓慢生长;最适生长pH为8,当pH<5或>10时,该菌的生长均受到明显抑制;M.esteraromaticum发酵产物对污水具有很好的絮凝效果,最佳絮凝条件为絮凝剂浓度45 mg/L、0.5 mmol/L Fe ^(3+)作为助凝剂、pH4、温度20℃;M.esteraromaticum分泌的脂肪酶能够良好的降解多种油脂,对大豆油的降解率高达约70%,其最适降解反应温度为34℃。本研究结果为M.esteraromaticum在废弃物处理等方面的应用研究奠定了基础。We used the strain of Microbacterium esteraromaticum isolated from homemade wine to investigate its toleranceto different concentrations of methanol and different environmental pH,the characteristics of the flocculant and its abilitytodegrade different oils and fats.The experimental results showed the bacterium could grow slowly in methanol mediumat amaximum concentration of 10%;the optimum growth pH was 8,and the growth of the bacterium was significantly inhibitedat pH<5 or>10.The optimum flocculation conditions were 45 mg/L flocculant concentration,0.5 mmol/L Fe^(3+)as coagulant,pH 4 and 20℃.The lipase secreted by M.esteraromaticum was able to degrade a wide range of oils and fats withadegradation rate of up to 70%,the optimum degradation temperature was 34℃.Our results provide the basis for thesubsequent application of M.esteraromaticum in waste disposal.

关 键 词：酯香微杆菌 耐甲醇 絮凝剂 降解油脂 

分 类 号：TQ35[化学工程]