利用光谱反射技术监测镉对榆树叶片光合机构的影响  

Monitoring the Effect of Cadmium on the Photosynthetic Apparatus of Ulmus pumila Leaves by Spectral Reflection Technology

作　　者：柳洁 薛忠财 王欣然 代海龙 LIU Jie;XUE Zhong-cai;WANG Xin-ran(College of Teacher Education,Hebei Normal University for Nationalities,Chengde,Hebei 067000;College of Resources and Environmental Sciences,Hebei Normal University for Nationalities,Chengde,Hebei 067000;Innovative Research Center for Soil and Characteristic Plant Nutrition in Mountainous Areas of Northern Hebei,Chengde,Hebei 067000;Chengde Kaidi Testing Technology Service Co.,Ltd.,Chengde,Hebei 067000)

机构地区：[1]河北民族师范学院教师教育学院,河北承德067000 [2]河北民族师范学院资源与环境科学学院,河北承德067000 [3]冀北山区土壤与特色植物营养创新研究中心,河北承德067000 [4]承德凯地检测技术服务有限公司,河北承德067000 [5]承德晟博农业科技有限公司,河北承德067000

出　　处：《安徽农业科学》2024年第1期110-114,共5页Journal of Anhui Agricultural Sciences

基　　金：河北省重点研发计划项目(21326401D,21327409D);河北民族师范学院双碳研究专项(ST2021001)。

摘　　要：[目的]拓展光谱反射技术在监测和评价土壤-植物系统中重金属污染程度和植物生长状况的应用,进一步明确其生理生化机理。[方法]采用盆栽试验,研究不同镉浓度处理条件下榆树叶片的快速叶绿素荧光、反射光谱和镉离子含量的变化,以光合机构的变化作为衡量生理变化的指标,探讨利用光谱反射技术监测镉对榆树叶片光合机构的影响。[结果]榆树对镉具有一定的抗性,在镉处理条件下榆树不同部位的镉含量呈现出根>茎>叶的分布规律,但是当过量的镉进入叶片后,榆树叶片的快速叶绿素荧光诱导曲线形状发生明显变化,光合电子传递受到抑制,并对光合机构造成伤害,主要荧光参数φ_(Po)、ψ_(Eo)、RE_(o)/RC、PI_(abs)、PI_(total)随着镉处理浓度的增加不断降低,V_(J)、V_(I)、Mo、ABS/RC、TRo/RC、DIo/RC则显著增加。榆树叶片在可见光波段(500~680 nm)的反射率随着土壤中镉浓度的增加而升高,在近红外区(740~1000 nm)的反射率则呈先降低再升高的趋势。通过分析镉处理条件下榆树叶片典型植被指数与荧光参数的关系表明,PRI、mSR_(705)和mND_(705)可以准确、快速地反映镉胁迫后榆树叶片光合机构变化指标。[结论]光谱反射技术可以用于监测镉对榆树叶片光合机构的影响,也为后续开展卫星影像大面积分析提供理论基础和技术支持。[Objective]To expand the application of spectral reflection technology in monitoring and evaluating the degree of heavy metal pollution and plant growth status in soil-plant systems,and further clarify the physiological and biochemical mechanism.[Method]We investigated the changes of fast chlorophyll fluorescence,spectral reflection and cadmium concentration in Ulmus pumila leaves under different cadmium concentration treatment conditions in this study.Then,the effects of cadmium on the photosynthetic apparatus of Ulmus pumila leaves were monitored by spectral reflection technology.[Result]The results showed that the cadmium concentration in different parts of Ulmus pumila shows as“root>stem>leaves”under cadmium treatment,indicating that it has certain resistance to cadmium stress.However,the shape of chlorophyll fluorescence induction curve of Ulmus pumila leaves was changed significantly when excess cadmium enters the leaves,which indicated that the photosynthetic electron transport was inhibited by cadmium.The fluorescence parameters ofφ_(Po),ψ_(Eo),RE_(o)/RC,PI_(abs),PI_(total) are decreasing with the increase of cadmium concentration in the soil,while the V_(J),V_(I),Mo,ABS/RC,TRo/RC,DIo/RC increased significantly.Meanwhile,the spectral reflection of Ulmus pumila leaves in the visible light band(500-680 nm)increases with the increase of cadmium concentration in the soil,and the spectral reflection in the near-infrared region(740-1000 nm)shows a trend of first decreasing and then increasing.It demonstrate that PRI,mSR_(705) and mND_(705) can accurately and quickly reflect the change in photosynthetic apparatus of Ulmus pumila leaves after cadmium stress,by analyzing the relationship between the typical vegetation index and the fluorescence parameters of Ulmus pumila leaves under cadmium treatment.[Conclusion]It can be seen that the spectral reflection technology can be used to monitor the influence of cadmium on the photosynthetic apparatus of Ulmus pumila leaves,and also provide theoretical basis and t

关 键 词：榆树 植物修复 镉 光合机构 光谱分析 

分 类 号：S771.8[农业科学—森林工程]