基于SSR标记的广东黄皮种质资源遗传多样性分析及分子身份证构建  

Genetic Diversity Analysis and Molecular Identity Establishment of Clausena lansium Germplasm Resources from Guangdong Province Based on SSR Markers

作　　者：陆育生 彭程 常晓晓[1] 邱继水[1] 陈喆 陈慧琼 LU Yu-sheng;PENG Cheng;CHANG Xiao-xiao;QIU Ji-shui;CHEN Zhe;CHEN Hui-qiong(Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640)

机构地区：[1]广东省农业科学院果树研究所、农业农村部南亚热带果树生物学与遗传资源利用重点实验室、广东省热带亚热带果树研究重点实验室,广州510640

出　　处：《生物技术通报》2023年第12期187-199,共13页Biotechnology Bulletin

基　　金：2022年省级乡村振兴战略专项资金种业振兴项目(2022-NPY-00-036,2022-NBH-00-010);国家自然科学基金项目(31501729);广东省现代农业产业技术体系创新团队建设项目(2021KJ116,2022KJ116);国家热带植物种质资源库建设项目(NTPGRC)。

摘　　要：揭示广东黄皮种质资源的遗传多样性水平及亲缘关系,为黄皮种质资源的合理保护和高效利用提供参考依据。利用12对SSR引物分析广东黄皮种质资源的多态性,利用UPGMA进行聚类分析,通过数字和字母赋值编码构建分子身份证。12对引物在供试材料中共扩增出43个等位基因(Na),平均等位基因数3.583;期望杂合度(He)变幅为0.294-0.665,均值为0.524;多态性信息指数(PIC)变幅为0.291-0.642,均值为0.484;Shannon信息指数(I)变化范围为0.567-1.096,均值为0.913;UPGMA聚类可将84份种质划分为6个类群,并对每一份供试材料构建了分子身份证。广东黄皮种质资源具有较为丰富的遗传多样性,未严格按照地理来源进行聚类,通过赋值编码SSR扩增得到的基因型,构建了黄皮资源的分子身份证。This work aims to reveal the genetic diversity and relationship among Guangdong wampee germplasms,and provide reference for the effective protection and efficient utilization of wampee germplasm resources.The wampee germplasms from Guangdong province were examined using 12 pairs of SSR primers to determine their genetic diversity.UPGMA was utilized for cluster analysis,while numerical and letter codes were assigned to create molecular identity cards.The results showed that the 12 pairs of primers generated a total of 43 alleles(Na),with an average of 3.583 alleles per locus in the 84 analyzed materials.The expected heterozygosity(He)ranged from 0.294 to 0.665,with a mean of 0.524.The polymorphism information content(PIC)ranged from 0.291 to 0.642,with a mean of 0.484.The Shannon information index(I)ranged from 0.567 to 1.096,with a mean of 0.913.The 84 germplasms were divided into six groups based on UPGMA clustering,and molecular identity was established for each examined germplasm resource.Guangdong wampee germplasm resources have high genetic diversity,while their clustering was not strictly conducted by their geographical origins.Based on the SSR genotyping,a digital and letter-based coding method was used to construct the molecular identity card for wampee resources.

关 键 词：黄皮 种质资源 SSR 遗传多样性 分子身份证 

分 类 号：S666.6[农业科学—果树学]