珊瑚状猴头菇多糖的提取及其体外抗氧化活性分析  

Extraction of Polysaccharide from Hericium corallinum and Analysis on Its in vitro Antioxidant Activity

作　　者：涂晓媛 褚路路 王冕 陈炳智[1,2] 江玉姬[1,2] TU Xiao-yuan;CHU Lu-lu;WANG Mian;CHEN Bing-zhi;JIANG Yu-ji(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002;Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002)

机构地区：[1]福建农林大学食品科学学院,福州350002 [2]福建农林大学菌物研究中心,福州350002

出　　处：《生物技术通报》2023年第12期276-286,共11页Biotechnology Bulletin

基　　金：安职院重点基金(N202304);福建农林大学创新基金(KFA20116A)。

摘　　要：珊瑚状猴头菇是近几年栽培驯化成功的新品种,珊瑚状猴头菇多糖具有抗氧化等生理活性。以多糖提取率为指标,利用响应面法优化热水浸提珊瑚状猴头菇多糖的提取工艺,并通过紫外吸收光谱、傅里叶变换红外光谱、X-衍射初步表征珊瑚状猴头菇多糖,同时测定其体外抗氧化活性。珊瑚状猴头菇多糖最佳工艺参数为提取温度93℃、提取时间2.0 h、料液比1∶33(g/mL)、颗粒大小80目;表征结果显示珊瑚状猴头菇多糖含有少量蛋白质和核酸,具有多糖化合物的典型吸收峰和吡喃糖环结构,同时可能存在晶体和非晶体结构;体外抗氧化试验表明,珊瑚状猴头菇多糖对DPPH、ABTS^(+)、·OH自由基有良好的清除效果,其EC50值分别为0.663 mg/mL、0.767 mg/mL和0.952 mg/mL。珊瑚状猴头菇多糖具有良好的体外抗氧化活性,研究结果为进一步探究珊瑚状猴头菇多糖体内抗氧化活性及其他功效奠定研究基础。Hericium corallinum,a newly cultivated and domesticated species,has a high content of polysaccharides with physiologically active properties such as antioxidation.Using the polysaccharide extraction rate as a benchmark,the response surface methodology was utilized to optimize the hot water extraction process of H.corallinum polysaccharides(HCP).UV,FT-IR and X-ray diffraction were used to preliminarily characterize HCP,and its antioxidative activity in vitro was measured.The results showed that the optimal process parameters for extracting HCP were an extraction temperature of 93℃,an extraction time of 2.0 h,a material-to-liquid ratio of 1∶33(g/mL),and a particle size of 80 mesh.The characterization results showed that HCP contained a small amount of proteins and nucleic acids,with typical absorption peaks of polysaccharide compounds and pyranose ring structure,and showed the presence of crystalline and non-crystalline structures.In vitro antioxidant experiments showed that HCP had good scavenging effects on DPPH,ABTS^(+)and·OH radicals,and their EC50 values were 0.663 mg/mL,0.767 mg/mL and 0.952 mg/mL,respectively.HCP has good antioxidant activity,and the results lay a research foundation for further exploring the antioxidant activity and other effects of coralline HCP in vivo.

关 键 词：珊瑚状猴头菇多糖 提取工艺 响应面法 结构 抗氧化 

分 类 号：R284.2[医药卫生—中药学]