斑马鱼应用于肝损伤疾病模型的研究进展  

Research progress of zebrafish as a model of liver injury

作　　者：杨靖亚[1,2,3] 包哲隈 杨兰珠 喻文娟 武万强[1] YANG Jingya;BAO Zhewei;YANG Lanzhu;YU Wenjuan;WU Wanqiang(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306;Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai),Ministry of Agriculture,Shanghai 201306;National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai),Shanghai 201306;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海201306 [3]国家淡水水产品加工技术研发分中心(上海),上海201306 [4]上海海洋大学水产与生命学院,上海201306

出　　处：《安徽农业大学学报》2023年第6期957-963,共7页Journal of Anhui Agricultural University

基　　金：国家自然科学基金青年项目(82103844)资助。

摘　　要：由于斑马鱼细胞组成、同源基因,免疫反应,药物代谢等方面与人类相比具有高度保守性。再加上斑马鱼体积小,产量大,适合被基因编辑等特点,适合于高通量的药物筛选。所以斑马鱼和斑马鱼胚胎常被用来模拟发育缺陷或化合物诱导的肝脏疾病。对斑马鱼作为肝脏疾病模型的优点,以及斑马鱼模型在免疫反应,药物代谢等方面的与人类的相似性进行综述。归纳了目前一些针对不同因素开发的斑马鱼模型及其相关病理机制以及相关药物筛选,并简要分析了其与哺乳动物模型的差异及优势,以期对肝脏疾病的药物筛选有所帮助。Compared with human,zebrafish is highly conservative in cellular composition,homologous genes,immune response and drug metabolism.In addition,zebrafish is small in size and large in output,which is suitable for gene editing and high throughput drug screening.Therefore,zebrafish and zebrafish embryos are often used to simulate liver diseases induced by developmental defects or compounds.This paper introduces the advantages of zebrafish as a model of liver disease,and the similarities between zebrafish model and human in immune response,drug metabolism,etc.Some of the current zebrafish models developed for different factors and their related patho-logical mechanisms as well as related drug screening were summarized,and their differences and advantages with mammalian models were briefly analyzed,with a view to contributing to drug screening for liver diseases.

关 键 词：斑马鱼 肝损伤 肝脏结构 药物筛选 免疫系统 

分 类 号：R575.5[医药卫生—消化系统] R-332[医药卫生—内科学]