RBM43调控Slug mRNA抑制肝癌细胞转移的机制研究  

RBM43 Inhibits Liver Cancer Cell Metastasis by Regulating Slug mRNA

作　　者：孔繁君 殷淑芳[1] 张弘炜[1] KONG Fan-jun;YIN Shu-fang;ZHANG Hong-wei(Department of General Surgery,Jiangsu Cancer Hospital,Nanjing 210000,China)

机构地区：[1]江苏省肿瘤医院普外科,南京210000

出　　处：《南昌大学学报（医学版）》2023年第6期21-26,共6页Journal of Nanchang University：Medical Sciences

基　　金：江苏省卫健委医学科研项目(20200374)。

摘　　要：目的 探讨RBM43通过调控Slug mRNA的稳定性抑制肝癌细胞转移的作用。方法 使用于江苏省肿瘤医院接受肝癌根治性切除术的88例患者的病理组织样品及肝细胞癌(HCC)细胞系Huh-7作为实验材料。通过免疫组织化学和蛋白免疫印迹确定RBM43在HCC组织中的表达情况。分别用RBM43表达载体和空白对照载体质粒转染Huh-7细胞,获得过表达RBM43组和对照组细胞。采用实时定量聚合酶链反应(qPCR)和蛋白免疫印迹检测2组细胞中RBM43和Slug mRNA及蛋白的表达情况,通过CCK8、集落形成和伤口愈合实验、Transwell侵袭实验评估2组细胞的增殖、迁移和侵袭能力。结果 RBM43在肿瘤组织中IHC评分和肿瘤组RBM43蛋白的相对表达水平均低于癌旁组织(P<0.05);过表达RBM43组细胞的RBM43和Slug mRNA和蛋白的相对表达水平均显著低于对照组(P<0.05),且过表达RBM43组细胞的在传代接种后3、5和7 d时的细胞活力均显著低于同时期的对照组(P<0.001);过表达RBM43组细胞的克隆形成能力,划痕恢复速度和Transwell侵袭能力均显著低于对照组(均P<0.05)。结论 RBM43通过调控Slug发挥抑制HCC细胞增殖和迁移作用,可能成为HCC治疗的有效治疗靶点。Objective To explore the role of RBM43 in inhibiting hepatocellular carcinoma(HCC) metastasis by regulating the stability of Slug mRNA.Methods Pathological tissue samples from 88 patients who underwent radical resection for HCC at Jiangsu Cancer Hospital and Huh-7 cell line were used as the experimental materials.The expression of RBM43 in HCC tissues was determined by immunohistochemistry and Western blotting.Huh-7 cells were transfected with RBM43 expression vectors and empty plasmids to establish RBM43 overexpression group and control group,respectively.The expression of RBM43 and Slug mRNA and protein in HCC cells was detected by qRT-PCR and Western blotting,respectively.The proliferation,migration,colony formation and invasion capacities were measured by CCK8,colony formations,wound healing and Transwell assays,respectively.Results The IHC score and protein expression of RBM43 in tumor tissues were lower than those in para-cancer tissues(P<0.05).The expression levels of RBM43 and Slug mRNA and protein in RBM43 overexpression group were lower than those in the control group(P<0.05),and the cell viability in RBM43 overexpression group was lower than that in the control group at 3,5 and 7 days after inoculation(P<0.001).In addition,the colony formation capability,scratch wound healing speed and invasion ability in RBM43 overexpression group were lower than those in the control group(P<0.05).Conclusion RBM43 inhibits HCC cell proliferation and migration by regulating Slug mRNA.Therefore,it may be an effective target for HCC treatment.

关 键 词：RBM43 SLUG 肝癌 转移 

分 类 号：R735.7[医药卫生—肿瘤]