链霉素驱动微藻叶绿素荧光动力学的响应研究  

Dynamic Response of Chlorophyll Fluorescence in Microalgae Driven by Streptomycin

作　　者：何书晗 张洪波 欧阳添 施军琼 吴忠兴 HE Shuhan;ZHANG Hongbo;OUYANG Tian;SHI Junqiong;WU Zhongxing(School of Life Sciences,Southwest University/Key Laboratory of Eco-environment in Three Gorges Reservoir Region,Ministry of Education/Chongqing Key Laboratory of Plant Ecology and Resources in Three Reservoir Region,Chongqing 400715,China;Chongqing No.95 Middle School,Chongqing 400084,China;Zhongke Ruihua Ecological Technology Co.Ltd.,Wuhan 430070,China)

机构地区：[1]西南大学生命科学学院/三峡库区生态环境教育部重点实验室/重庆市三峡库区植物生态与资源重点实验室,重庆400715 [2]重庆第九十五初级中学校,重庆400084 [3]武汉中科瑞华生态科技股份有限公司,武汉430070

出　　处：《西南大学学报（自然科学版）》2024年第2期81-89,共9页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(42177055);重庆市研究生科研创新项目(CYS21106,CYS20105)。

摘　　要：为了探究微藻对链霉素的敏感性,通过叶绿素荧光动力学(OJIP)分析蓝藻-拟柱孢藻(Raphidiopsis raciborskii)和绿藻-四尾栅藻(Scenedesmus quadricauda)对链霉素的响应.研究结果表明,链霉素对拟柱孢藻和四尾栅藻的反应中心耗散的能量(DI0/RC)、单位光面积吸收的能量(ABS/CS0)和单位反应中心吸收的能量(ABS/RC)均有显著的促进作用,对拟柱孢藻单位反应中心捕获的用于还原QA的能量(TR0/RC)起抑制作用,且链霉素能够明显降低拟柱孢藻的光合驱动力.利用质量浓度为0.05~1.0 mg/L和1.0~20.0 mg/L的链霉素分别培养拟柱孢藻和四尾栅藻,其比生长率分别是对照组(0.00 mg/L)的0.74~0.25和1.19~0.51倍.拟柱孢藻丙二醛含量和超氧化物歧化酶活性明显随链霉素质量浓度的升高而降低,过氧化氢酶活性随链霉素质量浓度的升高而增加.四尾栅藻的过氧化氢酶活性、丙二醛含量和超氧化物歧化酶活性对链霉素质量浓度响应敏感度低.因此,本研究认为蓝藻对链霉素的敏感性比绿藻显著,主要原因是拟柱孢藻和四尾栅藻的能量分布存在显著差异.The response of Raphidiopsis raciborskii and Scenedesmus quadricauda on streptomycin was determined by OJIP chlorophyll fluorescence kinetics to explore the sensitivity of microalgae to streptomycin.The results showed that streptomycin significantly facilitate the DI0/RC,ABS/CS0 and ABS/RC in Raphidiopsis raciborskii and Scenedesmus quadricauda,inhibited TR0/RC,and markedly reduced the photosynthetic oxygen evolution and driving force in Raphidiopsis raciborskii.The specific growth rates of Raphidiopsis raciborskii(0.05~1.0 mg/L)and Scenedesmus quadricauda(1.0~20.0 mg/L)in streptomycin treatment group was 0.74~0.25 and 1.19~0.51 times as much as that of control(0.00 mg/L),respectively.This study found that the malondialdehyde and superoxide dismutase activities were decreased significantly,and catalase activity was increased in Raphidiopsis raciborskii with increase of streptomycin concentration.Malondialdehyde,superoxide dismutase and catalase activities in Scenedesmus quadricaudawere not significantly affectedby streptomycin.Therefore,our results suggested that cayanobacteria was more sensitive to streptomycin than green algae,the main reason is due to the significant difference on energy distribution between Raphidiopsis raciborskii and Scenedesmus quadricauda.

关 键 词：链霉素 瞬时叶绿素荧光诱导动力学曲线 光合机构 拟柱孢藻 四尾栅藻 

分 类 号：Q949.2[生物学—植物学] X171.1[环境科学与工程—环境科学]