不同菌种对呕吐毒素脱毒效果的研究  

Detoxification Effects of Different Strains on Deoxynivalenol

作　　者：毛定雨 李永祺 刘颖 焦小丽 MAO Dingyu;LI Yongqi;LIU Ying;JIAO Xiaoi(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University/Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,Tianjin 300392)

机构地区：[1]天津农学院动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室,天津300392

出　　处：《现代农业科技》2024年第1期176-179,共4页Modern Agricultural Science and Technology

基　　金：2021年天津市校级大学生创新创业训练计划项目(202110061173);天津市农业动物繁育与健康养殖重点实验室开放基金项目(2020zdkf01);天津“131”创新型人才团队“天津农学院功能性畜牧产品研发创新团队”建设项目(20180318);天津市科技重大专项与工程项目“蛋鸡健康养殖和蛋品质质量综合提升工程技术集成与示范”(18ZXBFN CO0310)

摘　　要：本文选择短小芽孢杆菌、枯草芽孢杆菌、地衣芽孢杆菌为脱毒菌种,研究3种菌种的上清液、菌悬液和细胞悬浮液对呕吐毒素(DON)的脱毒效果。选择脱毒效果较好的处理进行单因素试验设计,研究其在不同温度(35、45、55℃)、不同pH值(5、7、9)下的脱毒效果。结果表明,3种菌种的上清液、菌悬液、细胞悬浮液均对DON有脱毒作用,其中短小芽孢杆菌菌悬液、枯草芽孢杆菌上清液、地衣芽孢杆菌上清液的脱毒率最高,分别为54.74%、63.32%、57.68%;其最适温度分别为55、45、45℃,脱毒率分别达到74.35%、75.50%、83.51%,显著高于其他温度下的脱毒率(P<0.05);其最佳pH值分别为7、5、5,脱毒率分别达到72.89%、70.81%、75.60%,显著高于其他pH值下的脱毒率(P<0.05)。This paper selected Bacillus pumilus,Bacillus subtilis,and Bacillus licheniformis as the detoxification strains to study the detoxification effects of the supernatants,bacterial suspensions,and cell suspensions of the three strains on deoxynivalenol(DON),selected a treatment with better detoxification effect and conduct a single factor experi-mental design to study its detoxification effect at different temperatures(35,45,55℃)and pH values(5,7,9).The results showed that the supernatants,bacterial suspensions,and cell suspensions of the three bacterial strains all had detoxi-fication effects on DON.Among them,Bacillus pumilus suspension,Bacillus subtilis supernatant,and Bacillus licheni-formis supernatant had the highest detoxification rate,which reached 54.74%,63.32%,and 57.68%,respectively,the optimal temperatures were 55,45,45℃,and the detoxification rates reached to 74.35%,75.50%,and 83.51%,respectively,which were significantly higher than those of other temperatures(P<0.05);the optimal pH values were 7,5,and 5,respectively,and the detoxification rate reached to 72.89%,70.81%,and 75.60%,which were significantly higher than those of other pH values(P<0.05).

关 键 词：呕吐毒素 短小芽孢杆菌 枯草芽孢杆菌 地衣芽孢杆菌 脱毒效果 

分 类 号：S816[农业科学—饲料科学]