牡丹雄蕊对慢性非细菌性前列腺炎大鼠模型的影响  

Effect of Paeonia suffruticosa Andr.Stamens on Rat Model of Chronic Abacterial Prostatitis

作　　者：程丹丹 高德民[2] 王岱杰 梁栋 庞志勇 徐亚振 王惠敏 袁文鹏 CHENG Dandan;GAO Demin;WANG Daijie;LIANG Dong;PANG Zhiyong;XU Yazhen;WANG Huimin;YUAN Wenpeng(Heze Branch,Qilu University of Technology(Shandong Academy of Sciences),Biological Engineering Technology Innovation Center of Shandong Province,Heze 274000,China;School of Pharmacy,Shandong University of TCM,Jinan 250355,China;Heze Peony Industrial Technology Research Institute,Heze 274000,China)

机构地区：[1]齐鲁工业大学(山东省科学院),山东省科学院菏泽分院,山东省生物工程技术创新中心,山东菏泽274000 [2]山东中医药大学药学院,山东济南250355 [3]菏泽牡丹产业技术研究院,山东菏泽274000

出　　处：《食品工业科技》2024年第2期342-348,共7页Science and Technology of Food Industry

基　　金：山东省重点研发计划-药食同源特色产品创新研发与应用示范(2021SFGC1205);科教产融合试点工程重大创新专项-鲁西南特色植物资源全链条关键技术研发及应用示范(2022JBZ02-06);甘肃临夏州国家级牡丹基因库建设及推广应用(YDZX2021103)。

摘　　要：为探究牡丹雄蕊对大鼠慢性非细菌性前列腺炎的药理作用。实验以去势+皮下注射激素(苯甲酸雌二醇0.25 mg·kg^(-1))诱导的慢性非细菌性前列腺炎大鼠为动物模型。将40只非细菌性前列腺炎模型大鼠随机分成5组(每组8只):模型组、阳性组(塞来昔布250 mg·kg^(-1))和牡丹雄蕊低、中、高剂量组(100、200、400 mg·kg^(-1))。另外选取等量生理盐水处理的正常大鼠作为空白对照组。以大鼠卵磷脂小体密度、前列腺指数及前列腺组织中细胞因子及血液中TXB2和6-K-PGF1α的表达水平为评价指标,评价牡丹雄蕊对慢性非细菌性前列腺炎的治疗作用。实验结果显示大鼠前列腺组织炎症病变程度明显改善,与模型组比较牡丹雄蕊各剂量组白细胞数目、大鼠前列腺重、前列腺指数、TXB2以及大鼠前列腺组织中PGE2的含量均明显降低(P<0.01,P<0.05);牡丹雄蕊高剂量组ICAM-1、INF-γ、TNF-α、MCP-1以及IL-8的含量显著降低(P<0.01,P<0.05),低剂量组也均有下降,但无显著差异(P>0.05);牡丹雄蕊高剂量中6-keto-PGF1α及NO的含量显著升高(P<0.01,P<0.05),低剂量组也均有升高,但无差异差异(P>0.05)。本研究表明牡丹雄蕊对慢性非细菌性前列腺炎具有治疗作用,为牡丹雄蕊的进一步开发应用提供了理论依据。In order to investigate the pharmacological effect of Paeonia suffruticosa Andr.stamens on chronic non-bacterial prostatitis(CNBP)in rats,the rats with chronic abacterial prostatitis induced by castration combined subcutaneous injection of hormone (estradiol benzoate 0.25 mg·kg^(-1)) were used as models. Forty rats with CNBP were randomly divided into five groups (8 per group): Model group (normal saline), positive group (celecoxib 250 mg·kg^(-1)), various doses of lyophilized powder of Paeonia suffruticosa Andr. stamens groups (100, 200, 400 mg·kg^(-1)). Another group of normal mice treated with normal saline as normal group. The therapeutic effect of Paeonia suffruticosa Andr. stamens on CNBP was evaluated by lecithin body density, prostatic index, cytokines in prostate tissue and the expression levels of TXB2 and 6-K-PGF1α in blood. The results showed that the inflammation of prostate tissue in rats was significantly improved. Compared with model group, the number of white blood cells, prostate weight, prostate index, TXB2 and the content of PGE2 in prostate tissue of rats were significantly decreased in each dosage group of Paeonia suffruticosa Andr. stamens (P<0.01, P<0.05). The cont-ents of ICAM-1, INF-γ, TNF-α, MCP-1 and IL-8 in high-dose group of Paeonia suffruticosa Andr. stamens were signi-ficantly decreased (P<0.01, P<0.05), and in low-dose group were also decreased, but there was no significant difference (P>0.05). The contents of 6-keto-PGF1α and NO in high dose of Paeonia suffruticosa Andr. stamens were significantly increased (P<0.01, P<0.05), and in low dose group were also increased, but there was no significant difference (P>0.05). This study showed that Paeonia suffruticosa Andr. stamens had therapeutic effect on chronic abacterial prostatitis, which would provide a theoretical basis for further development and application of the Paeonia suffruticosa Andr. stamens.

关 键 词：牡丹雄蕊 慢性非细菌性前列腺炎 苯甲酸雌二醇 炎症因子 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]