加利地韦对狂犬病病毒体外抑制作用研究  

Inhibitory effects of Galidesivir (BCX4430) on rabies virus in vitro

作　　者：谢渊 迟瑛琳 陶晓燕[1] 于鹏程[1] 刘茜 刘淑清[1] 朱武洋[1] XIE Yuan;CHI Ying-lin;TAO Xiao-yan;YU Peng-cheng;LIU Qian;LIU Shu-qing;ZHU Wu-yang(Key Laboratory of Medical Virology,Ministry of Health,National Institute for Viral Disease Control and Prevention,NHC Key Laboratory of Biosafety,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,卫健委生物安全重点实验室,北京102206

出　　处：《中国人兽共患病学报》2023年第12期1151-1157,1195,共8页Chinese Journal of Zoonoses

摘　　要：目的 研究加利地韦(Galidesivir, BCX4430)对不同毒力狂犬病病毒的体外抑制作用。方法 以法匹拉韦(T-705)和利巴韦林作为阳性对照,利用直接免疫荧光(DFA)、实时荧光定量PCR和半数组织培养感染剂量法(TCID50)等方法分析BCX4430对狂犬病病毒的体外抑制作用。结果 CVS-11和SC16感染N2a细胞和BHK-21细胞后,250μmol/L BCX4430均表现出较好的病毒抑制效果,与相应浓度利巴韦林的抑制效果相当,与相应浓度的T-705阳性对照相比,250μmol/L BCX4430处理后的病毒滴度(F=273.48,P<0.01)及mRNA拷贝数均显著降低(F=268.39,P<0.01)。结论 体外条件下,BCX4430可以显著抑制狂犬病病毒的复制。This study analyzed the inhibitory effects of BCX4430 on rabies virus with different virulence(CVS-11 and SC16)in vitro.Direct immunofluorescence(DFA),quantitative real-time PCR and half tissue culture infective dose(TCID 50)assays were used.BCX4430(250μmol/L),compared with T-705,significantly decreased the viral titer and the relative expression level of viral mRNA,similarly to the inhibitory effects of ribavirin.Thus,our study demonstrated that BCX4430 significantly inhibits the replication of rabies virus in vitro.

关 键 词：加利地韦 狂犬病病毒 体外抑制作用 

分 类 号：R373.9[医药卫生—病原生物学]