高效液相色谱法同时测定保健食品中的10种黄酮类化合物  

Simultaneous determination of 10 flavonoid compounds in health foods by high performa anc

作　　者：胡潇潇 洪月玲[1] 杨妍 齐斌 程名玉 董宁宁 HU Xiao-xiao;HONG Yue-ing;YANG Yan;QI Bin;CHENG Ming-yu;DONG Ning-ning(Zhengzhou Municipal Center for Disease Control and Prevention,Henan 450000,China)

机构地区：[1]郑州市疾病预防控制中心,河南450000

出　　处：《中国卫生检验杂志》2023年第22期2722-2725,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的研究提取溶剂和提取方式等条件对实验结果的影响,最终建立保健食品中大豆苷、大豆苷元、染料木苷、染料木素、芦丁、茨菲醇、芹菜素、松属素、柯因、高良姜等10种黄酮类化合物的高效液相色谱同时分析方法。方法样品经80%甲醇水常温超声提取30min后,将提取液在8000r/min条件下离心10min,取上清液过0.22μm微孔滤膜,用Agilent ZORBAX Eclipse XDB C_(18)(250mm×4.6mm,5μm)色谱柱分离,以甲醇-0.1%甲酸水为流动相梯度洗脱,流速为1.0ml/min,柱温为40℃,采用二极管阵列检测器在250nm、260nm270nm、289nm波长分别检测,外标法定量,并将此法应用于实际样品测定。结果10种黄酮类化合物分离度良好,线性范围为0.02μg/ml~100μg/ml,线性相关系数>0.999,方法检出限在0.002g/kg~0.020g/kg,样品加标回收率在85.9%~109%,相对标准偏差小于6.0%,稳定性满足实验要求。结论该方法操作简单,重复性好,灵敏度高,快速高效,可实现40min内完全出峰,且分离度和峰形良好,适用于保健食品中10种黄酮类化合物的同时测定。Objective This paper aims to study the effect of extraction solvent and extraction method on the experimental results,and establish a method for the simultaneous determination of 10 flavonoid compounds including daidzin,daidzein,genistin,genistein,rutin,camphenylol,apigenin,pinocembrin,chrysin,galangal in health foods by high performance liquid chromatography(HPLC).Methods The target analytes in sample were ultrasonically extracted with 80%methanol-water at room temperature for 30 min.The extraction solution was centrifuged for 10 min at 8000 r/min,and the supernatant was col-lected through O.22μm microporous filter membrane,then separated on an Agilent ZORBAX Eclipse XDB C_(18) column(250 mm×4.6 mm,5μm)with methanol-0.1%formic acid water as mobile phase at the flow rate of 1.0 ml/min.The tem-perature of column was 40C.The target analytes were detected with a photodiode array detector at the wavelengths of 250 nm,260 nm,270 nm,289 nm and conducted for quantitative analysis by external standard method and applied to the practical deter-mination.Results Good separation of the 10 flavonoid compounds was observed,with the linearity of 0.02μg/ml-100μg/ml,the correlated coefficient>0.999,and the detection limits of0.002 g/kg-0.020 g/kg.The average recoveries of the method were in the range of 85.9%-109.0%,with the relative standard deviations(RSDs)less than 6.0%.The stability of the method meets the detection requirements.Conclusion This method has the advantages of simple operation,good repeat-ability,high sensitivity,high rapidity and efficiency.This method can separate all the target analytes within 40 min and has good chromatographic resolutions,which can be applied to the simultaneous determination of 10 flavonoid compounds in health foods.

关 键 词：保健食品 黄酮类化合物 高效液相色谱法 

分 类 号：O657[理学—分析化学]