人体前列腺特异性抗原携带含酮基-脱氧壬酮糖酸的N-聚糖  

Human Prostate-Specific Antigen Carries N-Glycans with Ketodeoxynononic Acid

作　　者：Wei Wang Tao Zhang Jan Nouta Peter Avan Veelen Noortje de Haan Theo M.de Reijke Manfred Wuhrer Guinevere S.M.Lageveen-Kammeijer 

机构地区：[1]Center for Proteomics and Metabolomics,Leiden University Medical Center,Leiden 2333 ZA,Netherlands [2]Copenhagen Center for Glycomics,Department of Cellular and Molecular Medicine,University of Copenhagen,Copenhagen DK-2200,Denmark [3]Department of Urology,Amsterdam University Medical Centers,Location Academic Medical Center,University of Amsterdam,Amsterdam 1105 AZ,Netherlands [4]Analytical Biochemistry,Groningen Research Institute of Pharmacy,Faculty of Science and Engineering,University of Groningen,Groningen 9747 AG,Netherlands

出　　处：《Engineering》2023年第7期119-131,I0005,I0006,共15页工程（英文）

基　　金：supported by the Chinese Scholarship Council;Cure for Cancer Foundation;Astellas Pharma B.V。

摘　　要：Ketodeoxynononic acid(Kdn)is a rather uncommon class of sialic acid in mammals.However,associations have been found between elevated concentrations of free or conjugated Kdn in relation to human cancer progression.Hitherto,there has been a lack of conclusive evidence that Kdn occurs on(specific)human glycoproteins(conjugated Kdn).Here,we report for the first time that Kdn is expressed on prostate-specific antigen(PSA)N-linked glycans derived from human seminal plasma and urine.Interestingly,Kdn was found only in an a2,3-linkage configuration on an antennary galactose,indicating a highly specific biosynthesis.This unusual glycosylation feature was also identified in a urinary PSA cohort in relation to prostate cancer(PCa),although no differences were found between PCa and nonPCa patients.Further research is needed to investigate the occurrence,biosynthesis,biological role,and biomarker potential of both free and conjugated Kdn in humans.

关 键 词：Ketodeoxynononic acid GLYCOSYLATION Prostate cancer Prostate-specific antigen 

分 类 号：R446.6[医药卫生—诊断学]