肠道菌群与胃肠道炎症和消化道早癌的相关性研究  

Study on the correlation between intestinal flora and gastrointestinal inflammation and gastrointestinal early cancer

作　　者：余钊 杜勇 程翌 吴鹏 高欣 YU Zhao;DU Yong;CHENG Yi;WU Peng;GAO Xin(Department of Gastroenterology,Taikang Tongji(Wuhan)Hospital,Wuhan 430050,Hubei Province,China;Department of Gastroenterology,The General Hospital of the PLA Central Theater Command,Wuhan 430064,Hubei Province,China)

机构地区：[1]泰康同济(武汉)医院消化内科,湖北武汉430050 [2]中国人民解放军中部战区总医院消化内科,湖北武汉430064

出　　处：《中国临床药理学杂志》2023年第24期3575-3579,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 探索肠道微生物在胃肠道炎症和消化道早癌症发生中的作用,并从多个因素进行分析。方法 纳入胃肠道炎症和消化道早癌症的患者作为试验组;选取同期健康受试者作为对照组。记录试验组和对照组的饮食和生活习惯等相关信息,用高通量测序技术对2组样本的肠道微生物组成进行检测,并用酶联免疫吸附法对大鼠肉瘤致病基因(Ras)、大肠杆菌腺瘤性息肉基因(APC)和甲基巴豆酰辅酶A羧化酶(MCC)的表达水平进行检测。结果 试验组和对照组Ras表达水平分别是(500.00±12.40)和(312.14±9.72) pg·mL^(-1),APC的表达水平分别是(734.25±23.60)和(487.50±16.47) pg·mL^(-1),MCC的表达水平分别是(434.18±12.50)和(212.36±8.42) pg·mL^(-1),试验组Ras、APC和MCC表达水平均显著高于对照组,差异均有统计学意义(均P<0.05);与对照组比较,试验组中普氏菌门(Prevotella)、拟杆菌门(Bacteroidetes)、粪杆菌门(Faecalibacterium)菌群的丰度均增多,差异均有统计学意义(均P<0.05)。试验组和对照组Ace指数分别为223.57±2.04和186.52±2.04,Chao指数分别为235.57±7.04和174.52±8.04,Simpson指数分别为4.93±0.64和3.50±0.50,Shannon指数的指标分别是0.27±0.05和0.54±0.13,试验组的Ace指数和Chao指数均大于对照组,差异均有统计学意义(均P<0.05)。多元回归分析结果显示年龄、体质量指数、饮食习惯、抗生素使用史、疾病史与肠道菌群存在一定的关联(均P<0.05)。结论 肠道微生物菌群的组成与胃肠道炎症和消化道早癌的发病风险密切相关。Objective To explore the role of intestinal microbiome in gastrointestinal inflammation and early cancer of digestive tract,and analyze it from multiple factors.Methods Patients with gastrointestinal inflammation and early gastrointestinal cancer were included as treatment group.Healthy volunteers were selected as control group.The diet,lifestyle and other relevant information of the treatment group and control group were recorded,and the intestinal microbial composition of the two groups of samples was compared by high-throughput sequencing technology.The expression levels of sarcoma-causing(Ras),adenomatous polyp coli(APC) and methylcrotonyl-CoA carboxylase(MCC) in rats were detected by enzyme-linked immunosorbent assay.Results The expression levels of Ras in treatment group and control group were(500.00±12.40) and(312.14±9.72) pg·mL^(-1);the expression levels of APC were(734.25±23.60) and(487.50±16.47) pg·mL^(-1),respectively;the expression levels of MCC were(434.18±12.50) and(212.36±8.42) pg·mL^(-1),respectively.The expression levels of Ras,APC and MCC in treatment groups were significantly higher than those in control group,with statistical significance(all P <0.05).Compared with control group,the abundance of Prevotella,Bacteroidetes and Faecalibacterium in treatment groups increased,and the differences were statistically significant(all P <0.05).Ace index of treatment group and control group were 223.57±2.04 and 186.52±2.04;Chao index were 235.57±7.04 and 174.52±8.04;Simpson index were 4.93±0.64 and 3.50±0.50,respectively.The indexes of Shannon index were 0.27±0.05 and 0.54±0.13,respectively.The Ace index and Chao index of treatment groups were higher than those of control group,and the differences were statistically significant(all P <0.05).The results of multiple regression analysis showed that age,body mass index,diet habit,antibiotic use history and disease history were correlated with intestinal flora(all P <0.05).Conclusion The composition of intestinal microflora is closely related

关 键 词：肠道微生物 胃肠道炎症 消化道早癌症 饮食习惯 基因突变 

分 类 号：R97[医药卫生—药品]