顺阿曲库铵上调miR-505表达对骨肉瘤Saos-2细胞增殖、凋亡的影响  

Effect of cisatracurium up-regulating miR-505 expression on proliferation and apoptosis of osteosarcoma Saos-2 cells

作　　者：张苗 董彦海 崔子慧 ZHNAG Miao;DONG Yanhai;CUI Zihui(Department of Anesthesiology,the NO.2 Hospital of Baoding,Baoding 071051,Hebei Province,China)

机构地区：[1]保定市第二医院麻醉科,河北保定071051

出　　处：《世界临床药物》2023年第11期1159-1166,共8页World Clinical Drug

基　　金：保定市科技计划项目(2141ZF012)。

摘　　要：目的探讨顺阿曲库铵对人骨肉瘤细胞Saos-2增殖、凋亡的影响及其可能作用机制。方法体外培养Saos-2细胞,随机分为对照组、顺阿曲库铵低、中及高剂量组。实时定量荧光定量聚合酶链反应检测微小RNA(micro RNA,miR)-505表达量;采用MTT、平板克隆形成实验和流式细胞术分别检测细胞增殖、克隆形成及凋亡。结果与对照组比较,顺阿曲库铵低、中及高剂量组细胞增殖抑制率、凋亡率以及miR-505表达量均升高,克隆形成数减少,且呈剂量依赖性(P<0.05);转染miR-505模拟物后,细胞增殖抑制率、凋亡率升高,克隆形成数减少(P<0.05);转染miR-505抑制剂与顺阿曲库铵联合作用后,细胞增殖抑制率、凋亡率降低,克隆形成数增多(P<0.05)。结论顺阿曲库铵可通过上调miR-505表达抑制Saos-2细胞增殖、克隆形成,并促进细胞凋亡。Objective To investigate the effects of cisatracurium on proliferation and apoptosis of human osteosarcoma cell Saos-2 and its possible mechanism.Methods Saos-2 cells were cultured in vitro and randomly divided into control group,cisatracurium low,medium and high dose groups.Real time quantitative polymerase chain reaction was used to detect the expression of micro RNA(miR)-505.MTT,plate clone formation experiment and flow cytometry were used to detect cell proliferation,clone formation and apoptosis,respectively.Results Compared with the control group,the cell proliferation inhibition rate,apoptosis rate and miR-505 expression were increased in cisatracurium low,medium and high dose groups,and the number of clone formation was decreased in a dose-dependent manner(P<0.05).After transfection of miR-505 mimics,the cell proliferation inhibition rate and apoptosis rate increased,and the number of clone formation was decreased(P<0.05).After transfection of miR-505 inhibitor combined with cisatracurium,the cell proliferation inhibition rate and apoptosis rate decreased,and the number of clone formation was increased(P<0.05).Conclusion Cisatracurium can inhibit the proliferation and cloning of Saos-2 cells and promote apoptosis by up-regulating the expression of miR-505.

关 键 词：顺阿曲库铵 骨肉瘤 微小RNA-505 细胞增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]