马铃薯休眠解除相关基因StNF-YA8的表达分析  

Expression Analysis of the StNF-YA8 Gene Associated with Dormancy Release in Potato(Solanum tuberosum)

作　　者：王霄 魏涵 李世贵 王凯彤 赵亮 张宁[1,2] 司怀军[1,2] WANG Xiao;WEI Han;LI Shi-Gui;WANG Kai-Tong;ZHAO Liang;ZHANG Ning;SI Huai-Jun(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou 730070,China;Qitai Wheat Experimental Station,Xinjiang Academy of Agricultural Sciences,Qitai 831800,China)

机构地区：[1]甘肃农业大学生命科学技术学院,兰州730070 [2]甘肃农业大学省部共建干旱生境作物学国家重点实验室,兰州730070 [3]新疆农业科学院奇台麦类试验站,奇台831800

出　　处：《农业生物技术学报》2024年第1期50-59,共10页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31960444);甘肃省科技重大专项(GZGG-2021-6);科技部对发展中国家科技援助项目(KY201901015)。

摘　　要：核因子Y(nuclear factor Y,NF-Y)是真核生物中分布较为广泛的一类转录因子,由NF-YA、NFYB和NF-YC三种亚基构成,NF-YA参与调控植物的生长发育及胁迫响应。本研究用qRT-PCR方法分析了10个马铃薯(Solanum tuberosum)StNF-YA基因在块茎休眠解除过程中的表达模式。结果表明,StNFYA8基因在块茎休眠解除过程中持续上调表达。进一步克隆了StNF-YA8基因(GenBank No.XM_015309982.1),其ORF为591 bp,编码196个氨基酸。构建了StNF-YA8蛋白的亚细胞定位载体,结果显示,StNF-YA8蛋白定位在细胞核和细胞膜上。组织特异性分析发现,StNF-YA8基因在不同组织间存在表达差异。生物信息学分析表明,马铃薯StNF-YA8蛋白含有CBFB保守结构域,在进化程度上与野生马铃薯(S.commersonii)亲缘性最高;StNF-YA8蛋白与StNF-YB3/5/10/14/17和StNF-YC1/4/5/8/9蛋白互作;该基因启动子序列中存在参与光响应、昼夜节律控制和激素响应相关的顺式作用元件。本研究为深入解析马铃薯StNF-YA8基因参与调节块茎休眠解除的分子机制提供了基础资料。Nuclear factor-Y(NF-Y)is a class of transcription factors widely distributed in eukaryotes.It composed of NF-YA,NF-YB and NF-YC subunits.NF-YA is involved in regulating plant growth and development,and stress response.In this study,qRT-PCR method was used to analyze the expression pattern of 10 StNF-YA genes of potato(Solanum tuberosum)in the process of tuber dormancy release.The results showed that the StNF-YA8 gene was consistently up-regulated in expression during dormancy release in tubers.The StNF-YA8 gene was further cloned(GenBank No.XM_015309982.1),and its ORF was 591 bp,encoding 196 amino acids.The subcellular localization vector of StNF-YA8 protein was constructed,and the results showed that StNF-YA8 protein was localized in the nucleus and cell membrane.Tissue-specific analysis revealed that the StNF-YA8 gene was differentially expressed between different tissues.The results of bioinformatics analysis showed that the potato StNF-YA8 protein contained a conserved CBFB domain,and had the highest evolutionary relationship with wild potato(S.commersonii);StNF-YA8 protein closely interacted with StNF-YB3/5/10/14/17 and StNF-YC1/4/5/8/9 proteins;there were cis-acting elements involved in light response,circadian rhythm control and hormone response in the promoter sequence of this gene.The results can provide a basis for the molecular mechanism analysis of potato StNF-YA8 gene involved in regulating tuber dormancy release.

关 键 词：马铃薯 休眠 表达分析 核因子YA8(StNF-YA8) 生物信息学分析 

分 类 号：S532[农业科学—作物学]