脑蛋白水解物干预VaD小鼠海马神经元凋亡及可能的机制  

The Interferingeffect and Possible Mechanism of Cerebroprotein Hydrolysate-Ionthe Apoptosis of Hippocampal Neurons in Mice with Vascular Dementia

作　　者：王素平[1] 武筱林 王帅[1] 宋涛[1] 庄洁[1] 兰小磊[3] WANG Su-ping;WU Xiao-lin;WANG Shuai;SONG Tao;ZHUANG jie;LAN Xiao-lei(Department of Neuro-rehabilitation,Qingdao Third People's Hospital,Qingdao Shangdong 266041,China;Institute of Integrative Medicine,Qingdao University Medical College,Qingdao Shandong 266021,China;Department of Neurosurgery,Affiliated Hospital of Qingdao University,Qingdao Shandong 266003,China)

机构地区：[1]青岛市第三人民医院神经康复科,山东青岛266041 [2]青岛大学医学部中西医结合中心,山东青岛266021 [3]青岛大学附属医院神经外科,山东青岛266003

出　　处：《中华养生保健》2024年第2期4-8,共5页CHINESE HEALTH CARE

基　　金：青岛市医药卫生科研指导项目(2022-WJZD104)。

摘　　要：目的本研究旨在探讨脑蛋白水解物-I(CH-I)干预血管性痴呆(VaD)小鼠海马神经元凋亡的作用及可能的机制。方法通过Y迷宫从70只雄性昆明小鼠中筛选出学习能力较好的小鼠60只,随机选择10只为假手术组,其余50只建立VaD小鼠模型,随机分为模型组,脑活素阳性药组,CH-I低、中、高剂量组[10、20、30 mg/(kg·d)体质量],连续给药4周。末次给药后,应用Y迷宫检测小鼠的学习记忆能力,采用HE染色观察小鼠海马区细胞的形态结构,TUNEL检测小鼠海马组织细胞凋亡,Western blot分析海马组织Akt、p-Akt、Bcl-2、Caspase-9(Casp-9)、Caspase-3(Casp-3)表达。结果脑蛋白水解物-I可改善VaD小鼠学习记忆能力,改善海马神经细胞的形态结构和凋亡,增强p-Akt、Bcl-2表达,同时抑制Casp-9和Casp-3表达。结论CH-I可能通过调节PI3K/Akt信号通路而抑制VaD小鼠海马神经细胞凋亡。Objective This paper aims to explore the interfering effect and and possible mechanismof Cerebroprotein Hydrolysate-I(CH-I)on hippocampal neurons in mice of vascular dementia(VaD).Methods 60 male KM micewith good learning ability were selected fromtotal of 70 male KM micethrough the Y maze.Then,10 mice were randomly selected as the sham operation group,and the remaining 50 mice were used to establish VaD mouse model.The successful modeled mice were randomly divided into model group,Cerebrolysin(CBL)positive drug,low-dose,medium-dose,and high-dose groups received CH-I intraperitoneal injection(10,20,30 mg/kg body weight)dailyfor continuous administration 4 weeks.After the last administration,the learning and memory ability of mice were measured using Y maze.The cell morphology and structure of hippocampal cellswere observed by HE staining andthe apoptosis detected by TUNEL to detect,Western blot was used to analysis he protein expressions of p-Akt,Bcl-2,Caspase-9(Casp-9),and Caspase-3(Casp-3)in hippocampus.Results CH-I can improve the learning and memory ability of VaD mice,improve the shape and structure and apoptosis of neurons in the hippocampus,enhance the expressions of p-Akt and Bcl-2,and simutanouslyinhibit Casp-9 and Casp-3 expressions.Conclusion CH-I might inhibit apoptosisof hippocampal neurons in VaD mice byregulatingthe PI3K/Akt signaling pathway.

关 键 词：脑蛋白水解物-I VAD 海马 凋亡 PI3K/AKT 小鼠 

分 类 号：R741.05[医药卫生—神经病学与精神病学]