人参皂苷Rg1对Fusarium solani诱导作用及相关酶活性的影响  

Effects of Ginsenoside Rg1 on Induction of Fusarium solani and Related Enzyme Activities

作　　者：张善铭 陈彦霏 冯时 田义新[1] 许永华 张连学 高洁 卢宝慧 ZHANG Shanming;CHEN Yanfei;FENG Shi;TIAN Yixin;XU Yonghua;ZHANG Lianxue;GAO Jie;LU Baohui(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;State Local Joint Engineering Research Center of Ginseng Breeding and Development,Changchun130118,China;College of Plant Protection,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]人参新品种选育与开发国家地方联合工程研究中心,长春130118 [3]吉林农业大学植物保护学院,长春130118

出　　处：《吉林农业大学学报》2023年第6期702-708,共7页Journal of Jilin Agricultural University

基　　金：国家自然科学基金青年基金项目(31701354);吉林省科技发展计划项目(20180311001YY,20180201003NY);“111基地”建设项目(D17014)。

摘　　要：从吉林省人参主产区分离人参根腐病样,获得菌株130株,经形态学及分子生物学鉴定,病原菌均为Fusarium solani。采用生长速率法、菌丝干重法、孢子萌发法,筛选出对人参根腐病病原菌最佳诱导作用的Rg1浓度,以及最适Rg1浓度条件下对防御相关酶活力的影响,为明确Fusarium solani参与人参连作障碍提供参考依据。根据筛选出的最适浓度设置试验组,测定过氧化物酶(POD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)和脂氧合酶(LOX)6种酶活性变化。Rg1质量浓度为5 mg/L时,培养4 d后菌丝生长速率为9.33 mm/d,菌丝干重为(946.11±12.73)mg(P<0.05),孢子萌发率(SGR)为35.17%(P<0.05);菌丝生长促进率为3.466%(P<0.05),菌丝干重促进率为9.59%,孢子萌发促进率为44.55%,对人参根腐病菌诱导作用最强,在此浓度诱导影响下的POD、CAT、LOX酶活性得到了显著提高(P<0.05)。Ginseng root rot samples were isolated from the main ginseng producing region of Jilin province,and 130 strains were obtained and identified as Fusarium solani by morphology and molecular biology.Using growth rate method,hyphal dry weight method and spore germination method,the optimal Rg1 concentration for inducing Fusarium solani were selected,and the effects of the optimal Rg1 concentration on the activity of defense-related enzymes were analyzed,which provides a reference basis for clarifying the participation of F.solani in ginseng continuous cropping obstacles.The experimental group was set up based on the optimal concentration screened out,and the activities of the 6 related enzymes,peroxidase(POD),polyphenol oxidase(PPO),catalase(CAT),phenylalanine ammonia lyase(PAL),lipoxygenase(LOX)and superoxide dismutase(SOD),were measured.When concentration of Rg1 was 5 mg/L,mycelial growth rate was 9.33 mm/d after 4 days of culture,spore germination rate(SGR)was 35.17%(P<0.05),and dry weight of mycelium was(946.11±12.73)mg(P<0.05),promotion rate of mycelial growth was 3.466%(P<0.05),promotion rate of spore germination was 44.55%,and promotion rate of hyphal dry weight was 9.59%.The strongest induction effect on F.solani was observed,and the activities of POD,CAT and LOX enzymes were significantly improved(P<0.05)under the induction effect of this concentration.

关 键 词：人参 RG1 腐皮镰孢菌 诱导作用 酶活性 

分 类 号：S567.51[农业科学—中草药栽培]