牛磺胆酸钠诱导的急性坏死性胰腺炎小鼠肠屏障功能的变化  

Changes of intestinal barrier function in mice with acute necrotizing pancreatitis induced by sodium taurocholate

作　　者：陈逸飞 孙思慎 张尧 周春华 邹多武 Chen Yifei;Sun Sishen;Zhang Yao;Zhou Chunhua;Zou Duowu(Department of Gastroenterology,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]上海交通大学医学院附属瑞金医院消化内科,上海200025

出　　处：《中华胰腺病杂志》2023年第6期431-436,共6页Chinese Journal of Pancreatology

基　　金：国家自然科学基金(82270667)。

摘　　要：目的观察牛磺胆酸钠诱导的急性坏死性胰腺炎(ANP)小鼠肠道不同部位的病理学改变及肠屏障功能变化。方法18只雄性C57BL/6小鼠随机分为假手术组、ANP 24 h组、ANP 48 h组,每组6只。采用胰胆管逆行注射5%牛磺胆酸钠2μl/g建立ANP模型,假手术组仅进行插管手术操作。观察记录小鼠生存情况,采用苏木精-伊红染色观察各组小鼠胰腺、肠道组织病理形态,并进行病理学评分。使用全自动生化仪检测小鼠血清淀粉酶、脂肪酶水平,采用ELISA法检测血清D-乳酸水平,采用蛋白质免疫印迹法检测小肠组织紧密连接蛋白ZO-1、occludin表达。结果假手术组、ANP 24 h组、ANP 48 h组存活率分别为100%、36.4%、25.0%;胰腺病理评分分别为(0.67±0.82)、(10.58±0.64)、(8.81±1.55)分;血清淀粉酶水平分别为(479.14±86.42)、(5998.72±2096.31)、(3055.43±2336.5)U/L,脂肪酶水平分别为(18.56±3.84)、(558.20±559.65)、(112.58±94.91)U/L,ANP组胰腺病理评分、血清淀粉酶、脂肪酶均较假手术组升高,且ANP 24 h组升高更加显著,差异有统计学意义(P值均<0.05)。假手术组、ANP 24 h组、ANP 48 h组小肠上段病理评分分别为(0.17±0.41)、(2.11±1.41)、(1.61±0.80)分,小肠下段病理评分分别为(0.17±0.41)、(1.00±0.76)、(1.06±0.25)分,结肠病理评分分别为(0.33±0.52)、(0.67±0.82)、(0.67±0.52)分;血清D-乳酸水平分别为(388.92±126.30)、(2159.11±386.12)、(307.69±141.18)μmol/L;小肠上段ZO-1蛋白表达量分别为0.87±0.08、0.19±0.18、0.50±0.19,occludin蛋白表达量分别为0.98±0.04、0.13±0.08、0.69±0.04。ANP 24 h组、ANP 48 h组小肠上、下段病理评分较假手术组明显升高(P值均<0.05),而结肠病理评分差异无统计学意义;ANP 24 h组血清D-乳酸水平较假手术组显著升高(P<0.05),ANP 48 h组血清D-乳酸水平与假手术组无明显差异;ANP组肠道ZO-1、occludin蛋白表达量较假手术组均降低(P值均<0.05)。结论成功建立牛磺�Objective To observe the pathological changes of different intestinal parts and the changes of intestinal barrier function in mice with acute necrotizing pancreatitis(ANP)induced by sodium taurocholate.Methods A total of 18 male C57BL/6 mice were randomly divided into sham operation group,ANP 24 h group and ANP 48 h group with 6 mice in each group.The ANP model was established by retrograde injection of 2μl/g 5%sodium taurocholate into the pancreaticobiliary duct.The sham operation group only underwent intubation.The survival status was recorded.The pathology of pancreatic and intestinal tissues were observed using hematoxylin-eosin staining,and the pathological scores were evaluated.The activities of serum amylase and lipase were measured by automatic biochemistry analyzer.Serum D-lactate levels were detected by ELISA.The expression of tight-junction proteins ZO-1 and occludin in small intestinal tissue was detected by Western blotting.Results The survival rates of sham operation group,ANP 24 h group and ANP 48 h group were 100%,36.4%and 25.0%,respectively.The pancreatic pathological scores of sham operation group,ANP 24 h group and ANP 48 h group were(0.67±0.82),(10.58±0.64)and(8.81±1.55);the serum amylase activities were(479.14±86.42),(5998.72±2096.31)and(3055.43±2336.5)U/L;the serum lipase activities were(18.56±3.84),(558.20±559.65)and(112.58±94.91)U/L.The pancreatic pathological scores,serum amylase and lipase levels in ANP group were higher than those in sham operation group,and the increase in ANP 24 h group was more significant,and the difference was statistically significant(all P value<0.05).The upper small intestine pathological scores in different groups were(0.17±0.41),(2.11±1.41)and(1.61±0.80);The lower small intestine pathological scores were(0.17±0.41),(1.00±0.76)and(1.06±0.25);the colonic pathological scores were(0.33±0.52),(0.67±0.82)and(0.67±0.52),respectively.The serum D-lactic acid level was(388.92±126.30),(2159.11±386.12)and(307.69±141.18)μmol/L.The expression of ZO-1

关 键 词：胰腺炎 急性坏死性 牛磺胆酸钠 动物模型 肠屏障功能 

分 类 号：R576[医药卫生—消化系统]