细辛叶绿体基因组结构特征与系统进化分析  

Characteristics and phylogenetic analysis of chloroplast genome of medicinal plant Asari Radix et Rhizoma

作　　者：周军辉[1] 鬲晓敏 李莎莎 陈尘 贾芸 张明英 柏国清[1] ZHOU Jun-hui;GE Xiao-min;LI Sha-sha;CHEN Chen;JIA Yun;ZHANG Ming-ying;BAI Guo-qing(Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Xi'an Botanical Garden of Shaanxi Province(Institute of Botany of Shaanxi Province),Xi’an 710061,China;College of Pharmacy,Shaanxi University of Chinese Medicine/Key Laboratory for Research of"Qin Medicine"of Shaanxi Administration of Traditional Chinese Medicine,Xi’an 712046,China)

机构地区：[1]陕西省西安植物园(陕西省植物研究所),陕西植物资源保护与利用工程技术研究中心,陕西西安710061 [2]陕西中医药大学药学院,陕西省中医药管理局“秦药”研发重点实验室,陕西西安712046

出　　处：《中草药》2023年第24期8191-8199,共9页Chinese Traditional and Herbal Drugs

基　　金：陕西省科学院科技计划项目(2017K-12,2021K-10);西安市科技计划项目(20193021YF009NS009);西安市科学技术局“科技+”行动计划(20NYF0056);陕西省创新能力支撑计划(2022KJXX-27);陕西省自然科学基础研究计划项目(2022JM-458)。

摘　　要：目的以细辛药材为材料,采用高通量测序和生物信息学技术组装完整的叶绿体基因组序列,明确其结构特征、系统发育位置。方法基于Illumina测序平台对细辛叶绿体基因组序列进行结构特征分析;同时,采用最大似然法(maximum likelihood,ML)构建马兜铃科的系统发育关系。结果华细辛和辽细辛叶绿体基因组全长为160521~160555 bp,表现出典型的四分体结构,且两者叶绿体基因组GC含量均为38.5%。细辛叶绿体基因组包含128个基因,其中85个蛋白质编码基因,34个t RNA基因,8个r RNA基因。此外,系统发育分析结果显示,基于完整的叶绿体基因组和蛋白编码数据集构建的拓扑树一致,细辛属物种和马蹄香属物种形成一个支持率的单系分支。结论细辛叶绿体基因组测序、组装、结构特征分析,发现其大小介于Asarum bracteolata和A.sieboldii var.seoulense之间,IR区存在rpl16、rps19、ycf1、rpl23等基因扩张,研究结果丰富了细辛属植物遗传资源,为该属物种分子鉴定、系统发育以及野生资源保护与开发利用等研究提供理论基础。Objective The complete chloroplast genome of medicinal plant Xixin(Asari Radix et Rhizoma)sequenced and assembled by high-throughput sequencing and bioinformatics technology to confirm the genome structure characteristics,phylogenetic relationship and its evolutionary status.Methods The Illumina sequencing platform was used to perform characterization of the complete chloroplast genome sequence of Asari Radix et Rhizoma;Meanwhile,the maximum likelihood(ML)was used to construct the phylogenetic relationship of Aristolochiaceae.Results The results indicated that the whole length of Huaxixin(Asarum sieboldii Miq.)and Liaoxixin[A.heterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag.]plastid was 160521-160555 bp,showing a typical tetrad structure.The GC contents of the chloroplast genome were 38.5%.The plastid genome contains 128 genes,including 85 protein-coding genes,34 tRNA genes,and 8 rRNA genes.Furthermore,phylogenetic analysis indicated that topological trees recovered from the complete plastid genome and protein-coding datasets were consistent,suggesting that Asarum and Saruma species constitute a high support monophyletic clade.Conclusion Sequencing,assembly and structural analysis of Asari Radix et Rhizoma chloroplast genome in this study indicated that its size was between A.bracteolata and A.sieboldii var.seoulense,and rpl16,rps19,ycf1 and rpl23 genes were expanded in IR region.All research findings not only enriched the genetic resources of Asarum,but also provided a theoretical basis for the molecular identification,phylogeny,and protection,development and utilization of wild resources of this genus.

关 键 词：细辛 华细辛 辽细辛 叶绿体基因组 组装 系统发育 

分 类 号：R286.12[医药卫生—中药学]