微RNA-30d-5p通过调控ABCB5对胰腺癌进程的影响  

MiR-30d-5p affects the proliferation,invasion and metastasis of pancreatic cancer by regulating ABCB5

作　　者：郑坚江[1] 阿木提江·马合木提[1] 郭磊[1] 刘跃全[1] 张涛[1] ZHENG Jianjiang;AMUTIJIANG·Mahemuti;GUO Lei;LIU Yuequan;ZHANG Tao(Department of Pancreatic Surgery,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang Uygur Autonomous Region 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院胰腺外科,新疆维吾尔自治区乌鲁木齐830001

出　　处：《安徽医药》2024年第2期275-279,I0004,共6页Anhui Medical and Pharmaceutical Journal

基　　金：新疆少数民族科技人才特殊培养计划科研项目(2021D03019)。

摘　　要：目的 探讨微RNA(miR)-30d-5p在胰腺癌(PAAD)中的潜在作用和调控机制。方法 选择2016年12月至2019年1月来新疆维吾尔自治区人民医院接受手术治疗的胰腺癌病人35例,术后即刻获得胰腺肿瘤组织和癌旁非肿瘤组织,采用实时定量PCR检测miR-30d-5p在胰腺癌组织和细胞中的表达水平。使用细胞计数试剂盒(CCK-8)、克隆形成、transwell和流式细胞术检测miR-30d-5p对细胞增殖、迁移、侵袭和凋亡的影响。通过萤光素酶测定和蛋白质印迹法评估miR-30d-5p对ABCB5表达的调控作用。进一步构建裸鼠成瘤模型通过肿瘤质量和体积验证miR-30d-5p对肿瘤生长的影响。采用苏木精伊红染色验证肿瘤组织增殖情况。结果 与癌旁组织(1.03±0.17)及正常细胞(0.98±0.18)相比,胰腺癌组织(0.35±0.08)和细胞(0.63±0.08,0.23±0.07,0.48±0.07及0.31±0.05)中miR-30d-5p的表达显著降低(P<0.05);miR-30d-5p的过表达抑制了肿瘤细胞的活力、迁移和侵袭并促进了细胞凋亡且差异有统计学意义(P<0.05);抑制ABCB5的表达能抑制细胞活力、迁移和侵袭性并促进了胰腺癌的细胞凋亡且差异有统计学意义(P<0.05);体内实验证明miR-30d-5p组能够抑制肿瘤生长且差异有统计学意义(P<0.05)。结论MiR-30d-5p通过靶向ABCB5影响胰腺癌细胞增殖、迁移、侵袭和凋亡,可能是PC病人潜在的预后生物标志物及靶向治疗的靶点依据。Objective To explore the potential role and regulatory mechanism of microRNA(miR)-30d-5p in pancreatic adenocarci⁃noma(PAAD).Methods A total of 35 surgical cases of pancreatic cancer patients were selected from Xinjiang Uygur Autonomous Re⁃gion People's Hospital between December 2016 and January 2019 to investigate the immediate gain of pancreatic tumor tissue and ad⁃jacent carcinoma tissue.Real-time quantitative PCR was employed to detect the expression level of miR-30d-5p in pancreatic cancer tissues and cells.The effects of miR-30d-5p on cell proliferation,migration,invasion,and apoptosis were assessed using cell count⁃ing kit-8(CCK-8),colony formation assay,transwell assay,and flow cytometry.Luciferase assay and Western blot analysis were con⁃ducted to evaluate the regulatory effect of miR-30d-5p on ABCB5 expression.Furthermore,a nude mouse tumor model was established to verify the impact of miR-30d-5p on tumor growth by measuring tumor weight and volume.Hematoxylin and eosin staining was per⁃formed for histological examination.Results Compared with adjacent tissues(1.03±0.17)and normal cells(0.98±0.18),the expression of miR-30d-5p in pancreatic cancer tissues(0.35±0.08)and cells(0.63±0.08,0.23±0.07,0.48±0.07,and 0.31±005)was significantly decreased(P<0.05);overexpression of miR-30d-5p inhibited the vitality,migration,and invasion of tumor cells while promoting cell apoptosis with statistical significance(P<0.05);inhibition of ABCB5 expression suppressed cell vitality,migration,invasion,and in⁃duced apoptosis in pancreatic cancer cells with statistical significance(P<0.05);in vivo experiments demonstrated that the miR-30d-5p group could inhibit tumor growth with statistical significance(P<0.05).Conclusion MiR-30d-5p affects pancreatic cancer cell prolif⁃eration,migration,invasion,and apoptosis by targeting ABCB5.It may serve as a potential prognostic biomarker and target for targeted therapy in PC patients.

关 键 词：胰腺肿瘤 微RNA-30d-5p 细胞增殖 细胞侵袭 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]