LINC00310在胰腺癌中的高表达及其靶向功能预测  

High expression of LINC00310 in pancreatic cancer and prediction of its target function

作　　者：危丹明[1] 李建棣 谭洋 颜诗白 李建军[2] 黄志广 党裔武[1] 陈罡[1] 何娟 WEI Dan-Ming;LI Jian-Di;TAN Yang(Department of Pathology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学第一附属医院病理科,广西南宁530021 [2]广西医科大学第二附属医院普通外科

出　　处：《吉林医学》2024年第1期10-16,共7页Jilin Medical Journal

基　　金：广西壮族自治区自然科学基金[项目编号:2018GXNSFAA050051]。

摘　　要：目的:探讨长链间非编码RNA 310(LINC00310)在胰腺癌中的差异表达与其靶向功能的预测。方法:分析727例胰腺导管腺癌及548例周围正常胰腺组织对照样本中的LINC00310表达量。将LINC00310靶向的微小RNA(miRNA)与信使RNA(mRNA)分别与胰腺癌差异表达的miRNA与mRNA取交集后,使用Cytoscape v3.9.0构建长链非编码RNA(lncRNA)-miRNA-mRNA调控网络。利用“clusterProfiler”对LINC00310-miRNA-mRNA调控网络的基因功能进行注释分析。结果:LINC00310表达水平在胰腺癌中呈高表达,其标准平均差(SMD)值为0.17[0.06,0.29]。作为LINC00310预测调控的miRNA,miR-346,miR-596,miR-103a-2-5p,miR-661,miR-384及miR-15a-5p呈高表达,miR-4284及miR-1182呈低表达。根据miRNA预调控差异表达mRNA有46个(下调15个及上调31个)。LINC00310竞争性内源性RNA(ceRNA)可能参与磷脂酰肌醇3激酶/蛋白激酶B(PI3K-Akt)信号通路。结论:LINC00310作为临床胰腺癌诊断潜在分子标志物,可以为胰腺癌靶向治疗提供新的方案及思路。Objective To investigate the differential expression of LINC00310 in pancreatic cancer and the prediction of its target function.Method The expression of LINC00310 in 727 cases of pancreatic carcinoma and 548 samples of surrounding normal pancreatic tissue was analyzed.After intersection between the miRNAs and mRNAs targeted by LINC00310 and the miRNAs and mRNAs differentially expressed in pancreatic cancer,Cytoscape v3.9.0 was used to construct a lncRNA-miRNA-mRNA regulatory network.The gene function of that network was annotated and analyzed by"clusterProfiler".Results The expression level of LINC00310 was highly expressed in pancreatic cancer,and its SMD value was 0.17[0.06,0.29].As the miRNAs predicted to beregulated by LINC00310,miR-346,miR-596,miR-103a-2-5p,miR-661,miR-384 and miR-15a-5p were highly expressed,while miR-4284 and miR-1182 were underexpressed.According to the miRNA above,there were 46 differentially expressed mRNAs(15 down-regulated and 31 up-regulated).LINC00310 ceRNA may be involved in PI3K-Akt signaling pathway.Conclusion LINC00310,as a potential molecular marker for clinical diagnosis of pancreatic cancer,can provide new solutions and ideas for targeted therapy of pancreatic cancer.

关 键 词：胰腺癌 LINC00310 ceRNA 

分 类 号：R735.9[医药卫生—肿瘤]