京尼平苷通过PI3K/Akt通路促进糖尿病大鼠皮肤溃疡创面愈合  

Geniposide promotes skin ulcer wound healing in diabetic rats through the PI3K/Akt pathway

作　　者：姜雯雯[1] 刘欢[1] 陈晓燕[1] 荣小娟 刘燕玲[1] 曾威 JIANG Wenwen;LIU Huan;CHEN Xiaoyan;RONG Xiaojuan;LIU Yanling;ZENG Wei(School of Medicine,Jiangxi University of Science and Technology,Nanchang 330098,China)

机构地区：[1]江西科技学院医学院,南昌330098

出　　处：《中国比较医学杂志》2023年第12期14-20,共7页Chinese Journal of Comparative Medicine

基　　金：江西省中医药管理局科技计划项目(2023A0367);江西省卫生健康委科技计划项目(202311153);江西科技学院校级自然科学项目(23ZRZD05);江西省教育厅科学技术研究项目(GJJ202016)。

摘　　要：目的 探讨京尼平苷对糖尿病性皮肤溃疡大鼠的保护作用及其机制。方法 将大鼠分为正常组、模型组、京尼平苷治疗组(Gen(L):200 mg/kg;Gen(H):500 mg/kg)。在糖尿病大鼠皮肤溃疡造模后,各组大鼠均灌胃生理盐水或京尼平苷(n=6)。每天给药1次,每天记录各组伤口愈合情况及炎症情况。在糖尿病性皮肤溃疡治疗7 d后,分别通过测量皮肤溃疡创口面积、组化切片与TUNEL染色及Western blot蛋白免疫印迹法量化分析创面愈合、细胞凋亡及其相关调节蛋白表达的变化情况。结果 与模型组相比,京尼平苷(200 mg/kg和500 mg/kg)口服能显著促进糖尿病大鼠创面愈合,增加损伤处的收缩。在糖尿病大鼠皮肤创面凋亡研究中,京尼平苷治疗大鼠TUNEL染色阳性细胞明显减少(P<0.05)。京尼平苷能显著抑制皮肤炎症,促进创面修复可能与促进PI3K和Akt蛋白磷酸化有关。结论 京尼平苷通过抑制炎症反应和细胞凋亡促进糖尿病大鼠皮肤创面修复。Objective To investigate the protective effect of geniposide against diabetic rats with skin ulcer and the mechanism.Methods Rats were divided into a normal group,model group,and geniposide subgroups(Gen(L):200 mg/kg;Gen(H):500 mg/kg).Diabetic rats were treated with normal saline or geniposide by intragastric administration(n=6).Treatments were administered once a day,and the wound healing and inflammation of each group were recorded every day.After 7 days of treatment for diabetic skin ulcers,the wound area,tissue sections,TUNEL staining and Western blot were used to quantitatively analyze changes in wound healing,apoptosis,and related regulatory protein expression.Results Compared with the model group,the group receiving orally administered geniposide(200 and 500 mg/kg)showed significantly improved wound healing and increased contraction of the injured area.In terms of skin wound apoptosis in diabetic rats,TUNEL-positive cells were significantly reduced in geniposide subgroups(P<0.05).Geniposide significantly inhibited skin inflammation and promoted wound repair,which may be related to promotion of PI3K and Akt phosphorylation.Conclusions Geniposide promoted skin wound repair in diabetic rats by inhibiting inflammatory responses and apoptosis.

关 键 词：京尼平苷 糖尿病性皮肤溃疡 PI3K AKT 细胞凋亡 

分 类 号：R-33[医药卫生]