MNU诱导SD大鼠下焦湿热证尿血(膀胱癌)模型研究  

A model study of blood in urine(bladder cancer)during scorching humid fever in MNU-induced SD rats

作　　者：常拓 王洁 李楠 王天奇 CHANG Tuo;WANG Jie;LI Nan;WANG Tianqi(School of Traditional Chinese Medicine,Shenyang Medical College,Shenyang 110034;China.2.School of Traditional Chinese Medicine,Liaoning University of Traditional Chinese Medicine,Shenyang 110847;.3.School of Basic Medicine,Shenyang Medical College,Shenyang 110034;.4.Institute of Science and Technology,National Health Commission,Beijing 100081)

机构地区：[1]沈阳医学院中医药学院,沈阳110034 [2]辽宁中医药大学中医学院,沈阳110847 [3]沈阳医学院基础医学院,沈阳110034 [4]国家卫生健康委科学技术研究所,北京100081

出　　处：《中国比较医学杂志》2023年第12期49-54,共6页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划项目(2022YFF0711001);辽宁省科协科技创新智库项目(LNKX2022FH02);辽宁中医药大学博士后启动基金(21601A2090)。

摘　　要：目的 探讨N-甲基-N-亚硝基脲(MNU)诱导SD大鼠下焦湿热证尿血——膀胱癌(BC)模型的造模过程和成模率。方法 通过膀胱灌注MNU的方法在造模前、造模中、造模后共计6个时间点进行分批次样品采集,并对膀胱组织进行HE染色以了解膀胱原位癌的形成及发展过程。结果 实验过程顺利,在操作过程中大鼠未出现明显尿路损伤现象。通过本造模手法模型组大鼠膀胱内有明显的上皮增生、破损及大面积肿瘤形成和尿血现象。结论 MNU可以成功诱导SD大鼠下焦湿热证尿血模型(膀胱癌),且成模明显,死亡率低,实验数据可为膀胱癌模型的建立、改良和应用提供一定的参考。Objective To investigate the modeling process and model establishment rate of the N-methyl-N-nitrosourea(MNU)-induced lower jiao dampness-heat syndrome uremia bladder cancer(BC)model.Methods Batch samples were collected by bladder perfusion(MNU).HE staining and pathological observation of pathological tissues were carried out at six time points before,during,and after modeling to understand the formation and development of bladder carcinoma in situ.Results The experimental process was smooth,and no obvious urinary tract bleeding in rats occurred during the operation.Using this modeling technique,considerable bladder tumors were formed after week 8,and obvious epithelial hyperplasia,damage and large-scale tumor formation were observed in the bladder of rats in the model group.Conclusions MNU induces an SD rat bladder cancer model with obvious model establishment and low mortality.The experimental data provide a reference to establish,improve and apply the bladder cancer model.

关 键 词：N-甲基-N-亚硝脲 SD大鼠 膀胱癌 尿血 

分 类 号：R-33[医药卫生]