玉米自交系苗期根系形态鉴定及候选基因发掘  

Root Morphological Identification and Candidate Gene Discovery of Maize Inbred Lines at Seedling Stage

作　　者：张坤 王艺雄 杨金丹 张正 董春林 任志强[2] 王创云 常建忠 ZHANG Kun;WANG Yixiong;YANG Jindan;ZHANG Zheng;DONG Chunin;REN Zhiqiang;WANG Chuangyun;CHANG Jianzhong(College of Agriculture,Shanxi Agricultural University,Taiyuan 030031;Shanxi Institute of Organic Dry Farming Agriculture,Shanxi Agricultural University/Key Laboratory of Organic Dry Farming Agriculture of the Ministry of Agriculture and Rural Affairs(Jointly Built by Provinces and Departments)/Shanxi Provincial Key Laboratory of Organic Dry Farming,Taiyuan 030031)

机构地区：[1]山西农业大学农学院,太原030031 [2]山西农业大学山西有机旱作农业研究院/农业农村部有机旱作农业重点试验室(省部共建)/有机旱作山西省重点试验室,太原030031

出　　处：《植物遗传资源学报》2024年第1期84-96,I0005-I0007,共16页Journal of Plant Genetic Resources

基　　金：山西省重点研发计划(202102140601002-1);山西省科技重大专项计划子课题(202201140601025-1-03);山西省现代农业产业技术体系建设专项资金资助(2023CYJSTX01-09);中央引导地方发展资金项目(YDZJSX2022A036);山西农业大学生物育种工程(YZGC146)。

摘　　要：以134份玉米自交系为试验材料,对玉米的9个苗期根系性状进行表型鉴定,并利用分布于玉米基因组的44935个SNP标记,基于FarmCPU模型进行全基因组关联分析(GWAS,genome-wide association study)。结果表明9个根系性状表型变异范围在10.86%~55.96%之间,大部分表型间相关性达极显著水平(P<0.001),侧根长和总根长相关系数最高,为0.996,其次为侧根数与总根数达到0.993。共鉴定到32个显著关联的SNP位点(P=1.01e-11~9.74e-05),表型贡献率在0.54%~22.34%之间,主根长、总根长、最大根长、侧根长分别检测到4、8、3、9个显著的SNP位点;总根数、侧根数、不定根数分别检测到10、7、1个显著SNP位点;14个SNP位点同时与多个根系性状关联。12个显著关联位点位于已知根系相关性状QTL(Quantitative trait locus)区间内。共发掘49个根系候选基因,其中GRMZM2G028386(ABI4)、GRMZM2G135713(PUB23)、GRMZM5G870592(MYB98)、GRMZM2G156861(LOX1)、GRMZM2G160005(AUX16)、GRMZM2G126936(NAC2)等是重要的根系候选基因。本研究为克隆玉米根系发育相关基因,解析玉米根系发育分子机制提供了参考。In this study,134 maize inbred lines were used as experimental materials to identify phenotypic variations at nine root traits at seedling stage,followed by the genome-wide association study(GWAS)using FarmCPU model based on 44935 SNP markers.The results showed that the phenotypic variations at nine root traits ranged from 10.86%to 55.96%,and the correlation between most phenotypes reached a highly significant level(P<0.001).The correlation coefficient between lateral root length and total root length was the highest(0.996),followed by that between the number of lateral roots and total roots(0.993).A total of 32 significantly associated SNPs were identified(P value arranged from 1.01e^(-11)to 9.74e^(-5)),with phenotypic contribution rates ranging from 0.54%to 22.34%.Four,eight,three and nine significant SNPs were detected for main root length,total root length,maximum root length and lateral root length,respectively.Ten,seven and one significant SNPs were detected for total root number,lateral root number and adventitious root number,respectively.Fourteen SNPs were identified associating with multiple root traits.Twelve significant association sites were located within the intervals where root-related QTL(Quantitative trait locus)were reported.Forty-nine root-associated candidate genes were annotated,including formerly-identified GRMZM2G028386(ABI4),GRMZM2G135713(PUB23),GRMZM5G870592(MYB98),GRMZM2G156861(LOX1),GRMZM2G160005(AUX16),GRMZM2G126936(NAC2)and other important root candidate genes.Thus,this study provided reference for future cloning genes related to root development and analyzing the molecular mechanisms of root development in maize.

关 键 词：玉米自交系 根系 抗旱性 全基因组关联分析 基因挖掘 

分 类 号：S513[农业科学—作物学]