傅里叶变换红外光谱术用于急性及陈旧性心肌梗死的法医病理学诊断  

Forensic Pathological Diagnosis of Acute and Old Myocardial Infarction Using Fourier Transform Infrared Spectroscopy

作　　者：田甜 廖信彪[3] 张付[3] 邓恺飞 张吉 黄平 陈忆九 张建华 TIAN Tian;LIAO Xin-biao;ZHANG Fu;DENG Kai-fei;ZHANG Ji;HUANG Ping;CHEN Yi-jiu;ZHANG Jian-hua(Shanghai Key Laboratory of Forensic Medicine,Key Laboratory of Forensic Science,Ministry of Justice,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai 200063,China;School of Forensic Medicine,Shanxi Medical University,Jinzhong 030600,China;Key Laboratory of Forensic Pa-thology,Ministry of Public Security,Criminal Technology Center of Guangdong Provincial Public Security Department,Guangzhou 510050,China)

机构地区：[1]司法鉴定科学研究院上海市法医学重点实验室司法部司法鉴定重点实验室上海市司法鉴定专业技术服务平台,上海200063 [2]山西医科大学法医学院,山西晋中030600 [3]广东省公安厅刑事技术中心法医病理学公安部重点实验室,广东广州510050

出　　处：《法医学杂志》2023年第6期535-541,共7页Journal of Forensic Medicine

基　　金：国家重点研发计划资助项目(2022YFC3302002);国家自然科学基金资助项目(82072115);中央级公益性科研院所资助项目(GY2022G-3);上海市法医学重点实验室资助项目(21DZ2270800);上海市司法鉴定专业技术服务平台资助项目;法医病理学公安部重点实验室开放课题资助项目(GAFYBL202206)。

摘　　要：目的 利用傅里叶变换红外光谱术(Fourier transform infrared spectroscopy,FTIR)对不同病理变化阶段的心肌梗死组织进行光谱分析,以实现对急性和陈旧性心肌梗死的法医病理学诊断。方法 以苏木精-伊红(hematoxylin-eosin,HE)染色和免疫组织化学(immunohistochemistry,IHC)染色结果 为参考依据,采集冠状动脉粥样硬化性心脏病猝死组左心室前壁的早期缺血心肌、坏死心肌、心肌纤维化组织以及正常对照组心肌的FTIR数据,使用多元统计分析方法 进行数据分析。结果 对照心肌、早期缺血心肌和坏死心肌的平均归一化光谱较为相似,但平均二阶导数光谱存在较大差异,早期缺血心肌中各蛋白质二级结构的峰强度均明显高于其他类型心肌,坏死心肌α-螺旋的峰强度最低。心肌纤维化组织平均归一化光谱的酰胺Ⅰ和酰胺Ⅱ峰位明显向高波数方向移动,酰胺Ⅱ和酰胺Ⅲ的峰强度高于其他各类型心肌,平均二阶导数光谱在1 338、1 284、1 238和1 204 cm~(-1)处峰强度明显增强。主成分分析(principal component analysis,PCA)和偏最小二乘-判别分析(partial least square-discriminant analysis,PLS-DA)结果 显示,FTIR可区分不同类型的心肌组织。结论 FTIR技术具有对急性和陈旧性心肌梗死诊断的潜力,为心脏性猝死的死因分析提供了一种新的依据。Objective Fourier transform infrared spectroscopy(FTIR)was used to analyze myocardial infarction tissues at different stages of pathological change to achieve the forensic pathology diagnosis of acute and old myocardial infarction.Methods FTIR spectra data of early ischemic myocardium,necrotic myocardium,and myocardial fibrous tissue in the left ventricular anterior wall of the sudden death group of atherosclerotic heart disease and the myocardium of the normal control group were collected using hematoxylin-eosin(HE)and immunohistochemistry(IHC)staining as a reference,and the data were analyzed using multivariate statistical analysis.Results The mean normalized spectra of control myocardium,early ischemic myocardium and necrotic myocardium were relatively similar,but the mean second derivative spectra were significantly different.The peak intensity of secondary structure of proteins in early ischemic myocardium was significantly higher than in other types of myocardium,and the peak intensity of theα-helix in necrotic myocardium was the lowest.The peaks of amideⅠand amideⅡin the mean normalized spectra of myocardial fibrous tissue significantly shifted towards higher wave numbers,the peak intensities of amideⅡand amideⅢwere higher than those of other types of myocardium,and the peak intensities at 1338,1284,1238 and 1204 cm-1 in the mean second derivative spectra were significantly enhanced.Principal component analysis(PCA)and partial least square-discriminant analysis(PLS-DA)showed that FTIR could distinguish different types of myocardium.Conclusion FTIR technique has the potential to diagnose acute and old myocardial infarction,and provides a new basis for the analysis of the causes of sudden cardiac death.

关 键 词：法医病理学 傅里叶变换红外光谱术 急性心肌梗死 陈旧性心肌梗死 主成分分析 偏最小二乘-判别分析 

分 类 号：R89[医药卫生—法医学] DF795.1[医药卫生—临床医学] D919.1[政治法律—诉讼法学]