全血和血斑蛋白质组学差异分析  

Proteomic Difference Analysis of Whole Blood and Bloodstains

作　　者：黄傲 温姝博 孔倩倩 赵珍敏 刘希玲 HUANG Ao;WEN Shu-bo;KONG Qian-qian;ZHAO Zhen-min;LIU Xi-ling(School of Biology and Basic Medical Sciences,Soochow University,Suzhou 215123,Jiangsu Province,China;Shanghai Key Laboratory of Forensic Medicine,Key Laboratory of Forensic Science,Ministry of Justice,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai 200063,China)

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]司法鉴定科学研究院上海市法医学重点实验室司法部司法鉴定重点实验室上海市司法鉴定专业技术服务平台,上海200063

出　　处：《法医学杂志》2023年第6期549-556,共8页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81871534);中央级科研院所社会公益研究资助项目(GY2021G-2)。

摘　　要：目的 研究外周血形成干燥血斑后蛋白质水平的变化。方法 应用液相色谱-串联质谱法(LCMS/MS)和非标记定量(label-free quantification,LFQ)策略进行全血和血斑的蛋白质检测,分别采用R 4.2.1软件limma包和edgeR包筛选差异蛋白质,再进行生物学功能、信号通路和亚细胞定位分析。结果 全血和血斑中分别检测到623个和596个蛋白质,其中31个蛋白质的定量差异具有统计学意义,包括10个丰度在血斑中上调和21个丰度在血斑中下调的蛋白质。结论 全血和血斑中的蛋白质丰度高度相关,两者间蛋白质丰度的改变可能与细胞内源性蛋白和结构蛋白的变化相关。应用蛋白质组学技术可以辅助蛋白质生物标志物的筛选和鉴定,为法医学研究引入新型生物标志物。Objective To study the changes of protein levels in peripheral blood after it dried.Methods The proteins from whole blood and bloodstains were detected by liquid chromatography-tandem mass spectrometry(LC-MS/MS)and normalized by the label-free quantification(LFQ)method.The differen‐tial proteins were analyzed by using R 4.2.1 software,limma and edgeR package.The analysis of bio‐logical function,signaling pathway and subcellular localization for the differential proteins was then per‐formed.Results A total of 623 and 596 proteins were detected in whole blood and bloodstains,respec‐tively,of which 31 were statistically significant in the quantitative results,including 10 up-regulated and 21 down-regulated proteins in bloodstains.Conclusion The protein abundances in whole blood and bloodstains are highly correlated,and the variation of protein abundances may be related to the changes of endogenous and structural proteins in cells.The application of proteomics technology can assist the screening and identification of protein biomarkers,thereby introducing new biomarkers for fo‐rensic research.

关 键 词：法医学 蛋白质组学 液相色谱-串联质谱法 差异蛋白质 非标记定量策略 全血 血斑 

分 类 号：R89[医药卫生—法医学] DF795.1[医药卫生—临床医学] D919.1[政治法律—诉讼法学]