香菇多糖拮抗亚砷酸钠诱导小鼠肝脏脂质沉积的实验研究  

Experimental study of Lentinan antagonizes against hepatic lipid deposition induced by sodium arsenite in mice

作　　者：戴宗英 伍纤纤 杨渊 DAI Zongying;WU Qianqian;YANG Yuan(Guangxi Key Laboratory of Environmental Exposure Omics and Life Cycle Health,School of Public Health,Guilin Medical College,Guilin 541199,China)

机构地区：[1]广西全生命周期健康与保健研究重点实验室,桂林医学院公共卫生学院,广西桂林541199

出　　处：《公共卫生与预防医学》2024年第1期20-23,共4页Journal of Public Health and Preventive Medicine

基　　金：广西科技计划项目(桂科AD21220070);桂林医学院博士启动科研基金(20501020031)。

摘　　要：目的探讨香菇多糖对亚砷酸钠(sodium arsenite,SA)诱导小鼠肝脏脂质沉积的改善作用。方法以C57BL/6小鼠为实验对象,分别为对照组、SA染毒组、LNT+SA染毒组、LNT对照组。实验结束后收集血液和肝组织样本,应用酶联免疫吸附试验(ELISA)检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST)水平,肝组织一部分进行苏木精-伊红(HE)染色或油红O染色,观察肝脏病理损伤与脂质沉积特征,另一部分肝组织以ELISA法检测三酰甘油(TG)和脂联素(APN)水平。结果相比对照组或LNT对照组,SA染毒组血清AST、ALT含量升高,肝组织呈现病理损伤和脂质沉积特征,APN水平下降而TG水平升高(P<0.05)。相比SA染毒组,LNT+SA染毒组小鼠血清AST、ALT含量降低,肝组织病理损伤和脂质沉积程度明显减轻,APN水平上调而TG水平下调(P<0.05)。结论慢性SA染毒诱导C57BL/6小鼠肝功能损害、APN水平下调和脂质沉积,LNT干预明显改善SA诱导小鼠肝脏损伤和脂质沉积,可能与APN水平升高有关。Objective To investigate the ameliorative effect of Lentinan(LNT)on sodium arsenite(SA)-induced hepatic lipid deposition in mice.Methods C57BL/6 mice were used as the experimental subjects,which were divided into control group,SA-exposed group,LNT+SA-exposed group and LNT control group.Blood and liver tissue samples were collected at the end of the experiment,and serum glutathione transaminase(ALT)and glutathione aminotransferase(AST)levels were detected by enzyme-linked immunosorbent assay(ELISA).A part of liver tissues was stained with hematoxylin-eosin(HE)or oil red O to observe the characteristics of liver pathological damage and lipid deposition,and another part of liver tissues was used to detect triglyceride(TG)and Adiponectin(APN)levels by ELISA.Results Compared with control group or LNT control group,SA-exposed group showed the increased levels of AST and ALT,showing the characteristics of liver histopathological damage and lipid deposition,and the APN level decreased while the TG level increased(P<0.05).Compared with SA-exposed group,the levels of AST and ALT decreased in LNT+SA-exposed group,showing the reduced degree of liver tissue damage and lipid deposition,and APN level upregulated while TG level downregulated(P<0.05).Conclusion Chronic SA exposure induces liver function damage,APN downregulation and lipid deposition in C57BL/6 mice,while LNT intervention leads to the significantly improvement of hepatic damage and lipid deposition,which may be related to the elevated APN level in liver.

关 键 词：亚砷酸钠 肝脏脂质沉积 香菇多糖 拮抗效应 脂联素 

分 类 号：R114[医药卫生—卫生毒理学]