声流体对NE-4C神经干细胞的分化调控  

Differentiation regulation of NE-4C neural stem cells by acoustic streaming

作　　者：李文君 魏巍 康圣惠 段学欣 王艳艳 LI Wenjun;WEI Wei;KANG Shenghui;DUAN Xuexin;WANG Yanyan(State Key Laboratory of Precision Measuring Technology&Instruments,College of Precision Instruments and Optoelectronics Engineering,Tianjin University,Tianjin 300072,China)

机构地区：[1]精密测试技术与仪器国家重点实验室,天津大学精密仪器与光电子工程学院,天津300072

出　　处：《分析试验室》2023年第11期1429-1434,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(61971302)项目资助。

摘　　要：基于体声波(BAW)谐振器产生的千兆赫兹声流体(AS)剪切力,搭建了一种调控神经外胚层(NE-4C)干细胞分化进程的微芯片细胞调节系统,探究谐振器作用高度和AS作用时间对NE-4C干细胞神经球、轴突的形成以及轴突长度的影响。结果表明,通过降低谐振器作用高度,能够在增大AS流速的同时减小作用范围;在作用高度较低时,AS能够促进神经球的形成及转变;通过施加不同时间的AS激励,可以调节NE-4C干细胞在短时间内形成不同长度的神经突,并且在撤掉AS后,依旧能够促进轴突的伸长。在该系统下能够加快神经球的形成及神经突的伸长化,从而进一步促进NE-4C干细胞的分化。Based on the gigahertz acoustic streaming(AS)shear force produced by bulk acoustic wave(BAW)resonator,a microchip cell regulation system was built to regulate the differentiation process of neuroectodermal(NE-4C)stem cells,and to investigate the effects of the operating height of resonator and AS stimulation time on the formation of neurospheres and neurites and its length of NE-4C stem cells.The results showed that by reducing the operating height of resonator,the flow velocity of AS could be increased and the action range could be reduced simultanously.When operating height was low,the formation and transformation of neurospheres could be promoted by AS.By applying different AS stimulation time,neural stem cells could be adjusted to form neurites of different lengths in a short period of time,and the elongation of neurites could still be promoted after the AS was removed.Under this system,AS could accelerate the formation of neurospheres and the elongation of neurites,thus further promoting the differentiation of NE-4C stem cells.

关 键 词：体声波谐振器 声流体 神经干细胞 分化 

分 类 号：TH89[机械工程—仪器科学与技术]