复方红豆杉胶囊与替莫唑胺对胶质瘤细胞的协同杀伤作用  

Synergistic killing effect of compound taxus chinensis capsule and temozolomide on glioma cells

作　　者：张弛[1] 王英 杨福义[3] Zhang Chi;Wang Ying;Yang Fuyi(Jiamusi University,Jiamusi 154000;The Second Affiliated Hospital of Qiqihar Medical University,Qiqihar 161000;Department of Neurosurgery,The First Affiliated Hospital of Jiamusi University,Jiamusi 154000)

机构地区：[1]佳木斯大学,佳木斯154000 [2]齐齐哈尔医学院附属第二医院,齐齐哈尔161000 [3]佳木斯大学院附属第一医院神经外科,佳木斯154000

出　　处：《中国组织化学与细胞化学杂志》2023年第4期362-368,共7页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的探讨复方红豆杉胶囊(compound taxus chinensis capsule,CTCC)与替莫唑胺(temozolomide,TMZ)在抗胶质瘤的协同效应,并分析相关机制。方法体外培养人神经胶质瘤U251细胞,用MTT法检测CTCC与TMZ对U251细胞的IC50值。然后,将细胞分为四组:对照组、CTCC处理组(2 mg/mL),TMZ处理组(100μmol/L),CTCC+TMZ处理组。MTT实验检测各组细胞活力,用集落形成实验检测细胞集落形成能力,JC-1探针检测线粒体去极化,Annexin V/PI染色和TUNEL染色法检测细胞凋亡,免疫荧光观察KI-67和PCNA表达,Western blot检测细胞中Ki-67、PCNA、Bcl-2、Bax、细胞素色C和Cleaved Caspase-3水平。结果与对照组比较,其余3组细胞的活力、集落形成能力以及Ki-67、PCNA和Bcl-2表达均降低,线粒体去极化、凋亡以及Bax、细胞素色C和Cleaved Caspase-3表达均增加;其中CTCC+TMZ处理组较CTCC处理组和TMZ处理组变化更为明显。结论CTCC和CTCC均能抑制胶质瘤细胞的增殖并促进细胞凋亡,且二者联用具有协同效应,其机制可能与其二者对线粒体去极化以及Ki-67、PCNA、Bcl-2、Bax、细胞素色C和Cleaved Caspase-3表达的具有协同调控作用相关。Objective To investigate the synergistic effect of compound taxus chinensis capsule(CTCC)and temozolomide(TMZ)on anti-glioma,and to analyze the related mechanism.Methods Human glioma U251 cells were cultured in vitro,and the IC50 values of CTCC and TMZ on U251 cells were detected by MTT assay.Then,the cells were divided into four groups:control group,CTCC treatment group(2 mg/mL),TMZ treatment group(100μmol/L),and CTCC+TMZ treatment group.Cell viability was detected by MTT assay,colony formation ability was detected by colony formation assay,mitochondrial depolarization was detected by J-1 probe,apoptosis was detected by Annexin V/PI staining and TUNEL staining.The expressions of Ki-67 and PCNA were observed by immunofluorescence.The expressions of Ki-67,PCNA,Bcl-2,Bax,cytochromic C and Cleaved Caspase-3 were detected by Western blot.Results Compared with the control group,cell viability,colony formation,and Ki-67,PCNA,and Bcl-2 expressions were decreased in the other three groups,while mitochondrial depolarization,apoptosis,and Bax,cytochrom C,and Cleaved Caspase-3 expression were increased.The changes observed in the CTCC+TMZ treatment group were more pronounced than those in the CTCC group and the TMZ treatment group.Conclusion Both CTCC and CTCC can inhibit the proliferation and promote apoptosis in glioma cells,and their combination has a synergistic effect.The mechanism may be related to their synergistic regulation of mitochondrial depolarization and the expressions of KI-67,PCNA,Bcl-2,Bax,cytochromic C,and Cleaved Caspase-3.

关 键 词：胶质瘤 复方红豆杉胶囊 替莫唑胺 化疗敏感性 协同效应 

分 类 号：R739.41[医药卫生—肿瘤]