甲基转移酶样蛋白14对急性早幼粒白血病细胞增殖、凋亡、侵袭和迁移的调节作用  

Effects of METTL14 on proliferation,apoptosis,invasion and migration of acute promyelocytic leukemia cells

作　　者：张孟孟 伍燕平 张平平 王蒙 李佳佳[1] ZHANG Mengmeng;WU Yanping;ZHANG Pingping;WANG Meng;LI Jiajia(Department of Hematology,The First Affiliated Hospital of Bengbu Medical College,Bengbu 233003,China)

机构地区：[1]蚌埠医学院第一附属医院血液科,安徽蚌埠233003

出　　处：《山东医药》2024年第2期27-30,共4页Shandong Medical Journal

基　　金：安徽省自然科学基金项目(210805QH324)。

摘　　要：目的 探讨过表达和敲低甲基转移酶样蛋白14(METTL14)对急性早幼粒白血病细胞增殖、凋亡、侵袭和迁移的调节作用。方法 取人急性早幼粒白血病细胞NB4进行培养,分为过表达对照组、过表达组、干扰对照组、干扰组,过表达对照组及过表达组分别转染空白质粒和METTL14质粒,干扰对照组和干扰组分别转染si-NC和si-METTL14质粒。采用CCK-8法检测各组细胞增殖情况,流式细胞仪检测细胞凋亡情况,Transwell实验检测细胞侵袭和迁移能力,Dot Blotting法检测细胞N-6甲基腺苷(m6A)修饰水平。结果 与过表达对照组相比,过表达组细胞增殖率升高、细胞凋亡率降低、细胞迁移数、细胞侵袭数升高,NB4细胞m6A修饰水平提高(P均<0.05);与干扰对照组相比,干扰组细胞增殖率降低、细胞凋亡率增加、细胞迁移数、细胞侵袭数降低,NB4细胞m6A修饰水平降低(P均<0.05)。结论 METTL14可促进NB4细胞增殖、迁移、侵袭,其机制可能与提高m6A修饰水平有关。Objective To investigate the effects of overexpression and knockdown of methyltransferase-like protein 14(METTL14)on proliferation,apoptosis,invasion and migration of acute promyelocytic leukemia cells.Methods Human acute promyelocytic leukemia cells NB4 were cultured,and were divided into the overexpression control(Over-NC)group,overexpression group,interference control(si-NC)group,and interference group,respectively.The blank plasmid and METTL14 plasmid were transfected into NB4 cells in the control group and the overexpression group,respec-tively.The si-NC and si-METTL14 were transfected into NB4 cells in the interference control group and the interference group,respectively.Cell proliferation was detected by CCK-8,apoptosis was detected by flow cytometry,cell invasion and migration abilities were detected by Transwell chamber,and N-6 methyladenine(m6A)modification level was detect-ed by Dot blotting assay.Results Compared with the Over-NC group,the cell proliferation rate increased,apoptosis rate decreased,cell migration number and cell invasion number increased,and m6A modification level of NB4 cells in-creased in the overexpression group(all P<0.05).Compared with the si-NC group,the proliferation rate decreased,apop-tosis rate increased,cell migration number and cell invasion number decreased,and the m6A modification level of NB4 cells decreased in the interference group(all P<0.05).Conclusion METTL14 can promote the proliferation,migration and invasion of NB4 cells,and its mechanism may be related to the increase of m6A modification level.

关 键 词：急性早幼粒白血病 甲基转移酶样蛋白14 细胞增殖 细胞凋亡 细胞侵袭 细胞迁移 甲基转移酶 N6-甲基腺苷 

分 类 号：R733.71[医药卫生—肿瘤]