产活性氧假交替单胞菌GCY全基因组序列分析  

Whole-genome sequence analysis of Pseudoalteromonas sp.GCY with capability of producing reactive oxygen species

作　　者：岳昊 吴硕 王竞[1] 李泽龙 顾晨 YUE Hao;WU Shuo;WANG Jing;LI Zelong;GU Chen(School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,China;Power China Huadong Engineering Corporation Limited,Hangzhou 311122,China)

机构地区：[1]大连理工大学环境学院,辽宁大连116024 [2]中国电建集团华东勘测设计研究院有限公司,浙江杭州311122

出　　处：《大连理工大学学报》2024年第1期1-8,共8页Journal of Dalian University of Technology

基　　金：国家自然科学基金资助项目(21876018,42177375)。

摘　　要：生物源活性氧(reactive oxygen species,ROS)是环境ROS的重要来源,在生物元素地球化学循环中发挥着重要作用.但目前关于产ROS微生物的生物学信息尚不完善,影响微生物ROS产生的因素也未知.为此从近海沉积物中分离得到一株产胞外ROS的假交替单胞菌GCY(Pseudoalteromonas sp.GCY),利用全基因组测序表征了其潜在生物学功能.结果表明菌株GCY基因组大小为5.47 Mb,GC含量为43.39%,与胞外ROS产生相关的基因包括lodA、lodB和nqrA-F等.此外,氨基酸和Na+被证实影响胞外ROS的产生.进一步通过比较基因组分析,推测具备产胞外ROS能力的微生物在环境中广泛存在.综上,提供了产胞外ROS微生物全基因组分析报告,在分子水平上加深了对生物源ROS产生的理解,为开发利用各种环境中生物源ROS及认知生物源ROS的环境意义提供了重要视角.The biogenic reactive oxygen species(ROS)is an important source of ROS in the environment and plays an important role in the geochemical cycle of biogenic elements,but the biological information of ROS-producing microorganisms is not yet well available and the factors affecting ROS production by microorganisms are unknown.So the Pseudoalteromonas sp.GCY,an extracellular ROS-producing bacterium,is isolated from offshore sediments,and its potential biological functions are characterized by whole-genome sequencing.The result shows that the genome size of strain GCY is 5.47 Mb with GC content of 43.39%.The genes lod A,lod B and nqr A-F are involved in its extracellular ROS production while the amino acids and Na^(+)are confirmed to be influence factors for extracellular ROS production.Further,by comparative genomic analysis,it is hypothesized that microorganisms with extracellular ROS-producing ability are widely present in the environment.In summary,the whole-genome analysis report of extracellular ROS-producing microorganisms is provided,which deepens the understanding of biogenic ROS production at the molecular level and provides an important perspective for the exploitation of biogenic ROS in various environments and recognization of the environmental significance of biogenic ROS.

关 键 词：活性氧(ROS) 微生物 全基因组 影响因素 

分 类 号：X172[环境科学与工程—环境科学]