Naringin ameliorates H_(2)O_(2)-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway  

作　　者：Xiaomei Du Kexin Wang Xiaoyan Sang Xiangxing Meng Jiao Xie Tianxin Wang Xiaozhi Liu Qun Huang Nan Zhang Hao Wang 

机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology,Tianjin 300457,China [2]The key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guizhou 550025,China [3]Department of neurosurgery,the Fifth Central Hospital of Tianjin,Tianjin 300450,China [4]Tianjin Key Laboratory of Epigenetics for Organ Department in Preterm Infants,the Fifth Central Hospital of Tianjin,Tianjin 300450,China

出　　处：《Food Science and Human Wellness》2024年第3期1231-1245,共15页食品科学与人类健康（英文）

基　　金：supported by the open project of the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,China (GMU-2022-HJZ-06)。

摘　　要：Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties.In this study,PC12,HepG2 cells,and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms.The results showed that naringin inhibited H_(2)O_(2)-induced decline in cell viability and decreased,the content of reactive oxygen species in cells.Meanwhile,naringin prolonged the lifespan of flies,enhanced the abilities of climbing and the resistance to stress,improved the activities of antioxidant enzymes,and decreased malondialdehyde content.Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells.Moreover,naringin down-regulated the mRNA expressions of inr,chico,pi 3k,and akt-1,and up-regulated the mRNA expressions of dilp2,dilp3,dilp5,and foxo,thereby activating autophagy-related genes and increasing the number of lysosomes.Furthermore,the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling(IIS)pathway and activating the autophagy pathway,which was consistent with the result of network pharmacological predictions.

关 键 词：Drosophila melanogaster Insulin signaling(IIS)pathway NARINGIN PC12 cell HepG2 cell 

分 类 号：TS201.4[轻工技术与工程—食品科学]