Eugenol targeting CrtM inhibits the biosynthesis of staphyloxanthin in Staphylococcus aureus  

作　　者：Jiang Chang Bo Chen Zeqian Du Bowen Zhao Jiahui Li Ziyi Li Kannappan Arunachalam Ting Shi Dongqing Wei Chunlei Shi 

机构地区：[1]MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,State Key Laboratory of Microbial Metabolism,Shanghai Jiao Tong University,Shanghai 200240,China [2]School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Food Science and Human Wellness》2024年第3期1368-1377,共10页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China (31972169 and 32001798)。

摘　　要：Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public health.Especially the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of S.aureus treatment.Staphyloxanthin is a crucial virulence factor of S.aureus.Blocking staphyloxanthin production could help the host immune system counteract the invading S.aureus cells.In this study,we first screened for staphyloxanthin inhibitors using a virtual screening method.The outcome of the virtual screening method resulted in the identification of eugenol(300μg/mL),which significantly inhibits the staphyloxanthin production in S.aureus ATCC 29213,S.aureus Newman,MRSA ATCC 43300 and MRSA ATCC BAA1717by 84.2%,63.5%,68.1%,and 79.5%,respectively.The outcome of the growth curve assay,field-emission scanning electron,and confocal laser scanning microscopy analyses confirmed that eugenol at the test concentration did not affect the morphology and growth of S.aureus.Moreover,the survival rate of S.aureus ATCC 29213 and MRSA ATCC 43300 under H_(2)O_(2) pressure decreased to 51.9%and 45.5%in the presence of eugenol,respectively.The quantitative RT-PCR and molecular simulation studies revealed that eugenol targets staphyloxanthin biosynthesis by downregulating the transcription of the crtM gene and inhibiting the activity of the CrtM enzyme.Taken together,we first determined that eugenol was a prominent compound for staphyloxanthin inhibitor to combat S.aureus especially MRSA infections.

关 键 词：Staphylococcus aureus Staphyloxanthin EUGENOL Virtual screening 4 4’-Diapophytoene synthase(CrtM) 

分 类 号：TS201.3[轻工技术与工程—食品科学]