Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface  

作　　者：Pianpian Yan Ramachandran Chelliah Kyoung Hee Jo Xiuqin Chen Akanksha Tyagi Hyeon Yeong Jo Fazle Elahi Nam Chan Woo Min Seung Wook Deog Hwan Oh 

机构地区：[1]Department of Food Science and Biotechnology,College of Agriculture and Life Sciences,Kangwon National University,Chuncheon 24341,South Korea [2]Kangwon Institute ofInclusive Technology(KIIT),Kangwon National University,Chuncheon 24341,South Korea [3]Saveetha School of Engineering,(SIMATS)University,Sriperumbudur 600124,India [4]Seoulin Bioscience Company,Seongnam-si 13488,South Korea

出　　处：《Food Science and Human Wellness》2024年第3期1556-1566,共11页食品科学与人类健康（英文）

基　　金：supported by Brain Korea (BK)21 Plus Project (4299990913942)funded by the Korean Government,Korea;the Collabo Project funded by the Ministry of SMEs and Startups (C1016120-01-02);the National Research Foundation of Korea (NRF) (2018007551)。

摘　　要：Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.

关 键 词：Multi-resistant Staphylococcus aureus Metabolic profile SAEW BIOFILM Hurdle technology Electrode material 

分 类 号：TS201.3[轻工技术与工程—食品科学]