Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC  

作　　者：Yisheng Chen Xingjun Xi 

机构地区：[1]College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,China [2]Insitute of Food Nutrition and Safety,Shanxi Agricultural University,Taiyuan 030031,China [3]Institute of Agricultural Food Standardization,China National Institute of Standardization,Beijing 100191,China

出　　处：《Food Science and Human Wellness》2024年第3期1602-1607,共6页食品科学与人类健康（英文）

基　　金：financially supported by National Natural Science Foundation of China (21804058);Shanxi Postdoc Reward (SXBYKY2022001);Shanxi Scholarship Council of China (2021068);Shanxi Agricultural University High-Level Talent Project (2021XG013)。

摘　　要：As a widely used food preservative,methyl paraben was experimentally evidenced with serious hormonelike adverse effects.Herein,a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl paraben was proposed and validated in vinegar and coconut juice.First,the detectability of the bioautography to the analyte on different layer materials was estimated,revealing that normal silica gel was the best choice.After that,the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices.The separation result was then coupled to the optimized bioautography,enabling instant and straightforward screening of the targeted conpound.For accurate quantification,bioluninescent inhibition pattern caused by the analyte was processed by image analysis,giving useful sensitivity(LOD>16 mg/kg),precision(RSD<10.1%)and accuracy(spike-recovery rate 76.9%-112.2%).Finally,the suspected result was confirmed by determining its MS fingerprint,further strengthening the reliability of screening.

关 键 词：High performance thin-layer chromatography Bio-screening Bioluminescence Image analysis Methyl paraben 

分 类 号：TS202.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]