A novel AgNPs/MOF substrate-based SERS sensor for high-sensitive on-site detection of wheat gluten  

作　　者：Linglin Fu Yanzhuo Du Jinru Zhou Huan Li Minzi Wang Yanbo Wang 

机构地区：[1]Food Safety Key Laboratory of Zhejiang Province,School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China [2]Zhejiang Engineering Research Institute of Food&Drug Quality and Safety,School of Management and E-Business,Zhejiang Gongshang University,Hangzhou 310018,China [3]Zhejiang Dien Biotech Co.,Ltd.,Hangzhou 310030,China

出　　处：《Food Science and Human Wellness》2024年第2期681-687,共7页食品科学与人类健康（英文）

基　　金：financially supported by the Zhejiang Provincial Natural Science Foundation of China(LY21C200008)。

摘　　要：Gluten,known as the major allergen in wheat,has gained increasing concerns in industrialized countries,resulting in an urgent need for accurate,high-sensitive,and on-site detection of wheat gluten in complex food systems.Herein,we proposed a silver nanoparticles(AgNPs)/metal-organic framework(MOF)substrate-based surface-enhanced Raman scattering(SERS)sensor for the high-sensitive on-site detection of wheat gluten.The detection occurred on the newly in-situ synthesized AgNPs/MOF-modified SERS substrate,providing an enhancement factor(EF)of 1.89×10^(5).Benefitting from the signal amplification function of AgNPs/MOF and the superiority of SERS,this sensor represented high sensitivity performance and a wide detection range from 1×10^(-15)mol/L to 2×10^(-6)mol/L with a detection limit of 1.16×10^(-16)mol/L,which allowed monitoring the trace of wheat gluten in complex food system without matrix interference.This reliable sandwich SERS sensor may provide a promising platform for high-sensitive,accurate,and on-site detection of allergens in the field of food safety.

关 键 词：Wheat allergy GLUTEN Surface-enhanced Raman scattering Metal-organic framework DNA recognition 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.37[轻工技术与工程—食品科学与工程]