Transcriptomics integrated with metabolomics reveals the mechanism of CaCl_(2)-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts  

作　　者：Cui Li Shuhui Song Yanan He Haijie Liu 

机构地区：[1]College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China [2]Institute of Agri-food Processing and Nutrition,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China

出　　处：《Food Science and Human Wellness》2024年第2期801-812,共12页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(31972091)。

摘　　要：Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli sprouts.On the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW.Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades.Besides,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.

关 键 词：Broccoli sprouts CaCl_(2)-HCl electrolyzed water GLUCOSINOLATES TRANSCRIPTOMICS Metabolomics 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]