Palmitoleic acid on top of HFD ameliorates insulin resistance independent of diacylglycerols and alters gut microbiota in C57BL/6J mice  

作　　者：Qijian Liang Yan Zheng Fanli Meng Xiaofan Jiang Qingcai Zhen Zhongting Lu Shixiu Zhang Lei Du Hao Wu Xin Guo 

机构地区：[1]Department of Nutrition and Food Hygiene,School of Public Health,Cheeloo College of Medicine,Shandong University,Jinan 250012,China [2]Research Center of Translational Medicine,Jinan Central Hospital Affiliated to Shandong First Medical University,Jinan 250013,China [3]Department of Hepatology,Qilu Hospital of Shandong University,Jinan 250012,China [4]Institute of Hepatology,Shandong University,Jinan 250012,China

出　　处：《Food Science and Human Wellness》2024年第2期856-868,共13页食品科学与人类健康（英文）

基　　金：by National Natural Science Foundation of China(81803224);Young Scholars Program of Shandong University(2018WLJH33)to X.G.;National Natural Science Foundation of China(81973031);Cheeloo Young Scholar Program of Shandong University(21320089963054)to H.W.;Young Scholars Program of Shandong University(2018WLJH34);the Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology(LMDBKF-2019-05)to L.D.

摘　　要：With the prevalence of obesity and obesity-related metabolic syndrome,such as insulin resistance in recent years,it is urgent to explore effective interventions to prevent the progression of obesity-related metabolic syndrome.Palmitoleic acid is a monounsaturated fatty acid that is available from dietary sources,mainly derived from marine products.P almitoleic acid plays a positive role in maintaining glucose homeostasis and reducing inflammation.However,it is still unknow the mechanism of palmitoleic acid in ameliorating insulin resistance.Here,we investigated the effects of palmitoleic acid on chow diet(CD)-fed and high-fat diet(HFD)-fed mice,which were fed CD or HFD for 12 weeks before administration.We administrated mice with BSA(control),oleic acid,or palmitoleic acid for 6 weeks on top of CD or HFD feeding.We found that palmitoleic acid only improved glucose homeostasis in HFD-fed obese mice by increasing glucose clearance and reducing HOMA-IR.Further study explored that palmitoleic acid changed the composition of gut microbiota by decreasing Firmicutes population and increasing Bacteroidetes population.In colon,palmitoleic acid increased intestinal tight junction integrity and reduced inflammation.Moreover,palmitoleic acid decreased macrophage infiltration in liver and adipose tissue and increase glucose uptake in adipose tissue.Diacylglycerol(DAG)in tissue(for example,liver)is found to positively correlated with HOMA-IR.HFD enhanced the levels of DAGs in liver but not in adipose tissue in this study.Palmitoleic acid did not reverse the high DAG levels induced by HFD in liver.Therefore,in HFD-fed mice,palmitoleic acid reduced insulin resistance by an independent-manner of DAGs.It might be associated with the beneficial effects of palmitoleic acid on altering the gut microbiota composition,improving of intestinal barrier function,and downregulating the inflammation in colon,liver,and adipose tissue.

关 键 词：Palmitoleic acid High fat diet Insulin resistance Gut microbiota 

分 类 号：TS201.4[轻工技术与工程—食品科学]