One stone two birds:electrochemical and colorimetric dual-mode biosensor based on copper peroxide/covalent organic framework nanocomposite for ultrasensentive norovirus detection  

作　　者：Guobao Ning Quanmei Duan Huan Liang Huifang Liu Min Zhou Chunlan Chen Chong Zhang Hui Zhao Canpeng Li 

机构地区：[1]School of Chemical Science and Technology,Yunnan University,Kunming 650091,China [2]Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China [3]Kunming Customs Technology Center,Kunming 650228,China

出　　处：《Food Science and Human Wellness》2024年第2期920-931,共12页食品科学与人类健康（英文）

基　　金：financially supported by National Key Research and Development Program of China(2022YFC2601604);Major science and technology project of Yunnan Province(202202AE090085);the National Natural Science Foundation of China(32160597;32160236);Science and technology talent and platform plan of Yunnan;Key Scientific and Technology Project of Yunnan(202203AC100010);Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001);the second phase of“Double-First Class”program construction of Yunnan University;grants from State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University(2021KF005);Key Scientific and Technology Project of Yunnan(202002AE320005);Program for Excellent Young Talents of Yunnan University;the Program for Donglu Scholars of Yunnan University。

摘　　要：Norovirus(NoV)is regarded as one of the most common causes of foodborne diarrhea in the world.It is urgent to identify the pathogenic microorganism of the diarrhea in short time.In this work,we developed an electrochemical and colorimetric dual-mode detection for NoV based on the excellent dual catalytic properties of copper peroxide/COF-NH_(2)nanocomposite(CuO_(2)@COF-NH_(2)).For the colorimetric detection,NoV can be directly detected by the naked eye based on CuO_(2)@COF-NH_(2)as a laccase-like nonazyme using“peptide-NoV-antibody”recognition mode.The colorimetric assay displayed a wide and quality linear detection range from 1 copy/mL to 5000 copies/mL of NoV with a low limit of detection(LOD)of 0.125 copy/mL.For the electrochemical detection of NoV,CuO_(2)@COF-NH_(2)showed an oxidation peak of copper ion from Cu^(+)to Cu^(2+)using“peptide-NoV-antibody”recognition mode.The electrochemical assay showed a linear detection range was 1-5000 copies/mL with a LOD of 0.152 copy/mL.It's worthy to note that this assay does not need other electrical signal molecule,which provide the stable and sensitive electrochemial detection for NoV.The electrochemical and colorimetric dual-mode detection was used to detect NoV in foods and faceal samples,which has the potential for improving food safety and diagnosing of NoV-infected diarrhea.

关 键 词：NOROVIRUS Specific peptides Electrochemical and colorimetric assay DUAL-MODE Copper peroxide/covalent organic framework 

分 类 号：TS207.3[轻工技术与工程—食品科学] TP212[轻工技术与工程—食品科学与工程]