A core-satellite self-assembled SERS aptasensor containing a“biological-silent region”Raman tag for the accurate and ultrasensitive detection of histamine  

作　　者：Chen Chen Yingfang Zhang Ximo Wang Xuguang Qiao Geoffrey I.N.Waterhouse Zhixiang Xu 

机构地区：[1]Key Laboratory of Food Processing Technology and Quality Control in Shandong Province,College of Food Science and Engineering,Shandong Agricultural University,Tai’an 271018,China [2]School of Chemical Sciences,The University of Auckland,Auckland 1142,New Zealand

出　　处：《Food Science and Human Wellness》2024年第2期1029-1039,共11页食品科学与人类健康（英文）

基　　金：financially supported by the National Natural Science Foundation of China(31972149);funding support from the MacDiarmid Institute for Advanced Materials and Nanotechnology;the Dodd-Walls Centre for Photonic and Quantum Technologies。

摘　　要：Herein,a novel interference-free surface-enhanced Raman spectroscopy(SERS)strategy based on magnetic nanoparticles(MNPs)and aptamer-driven assemblies was proposed for the ultrasensitive detection of histamine.A core-satellite SERS aptasensor was constructed by combining aptamer-decorated Fe_(3)O_(4)@Au MNPs(as the recognize probe for histamine)and complementary DNA-modified silver nanoparticles carrying 4-mercaptobenzonitrile(4-MBN)(Ag@4-MBN@Ag-c-DNA)as the SERS signal probe for the indirect detection of histamine.Under an applied magnetic field in the absence of histamine,the assembly gave an intense Raman signal at“Raman biological-silent”region due to 4-MBN.In the presence of histamine,the Ag@4-MBN@Ag-c-DNA SERS-tag was released from the Fe_(3)O_(4)@Au MNPs,thus decreasing the SERS signal.Under optimal conditions,an ultra-low limit of detection of 0.65×10^(-3)ng/mL and a linear range 10^(-2)-10^5 ng/mL on the SERS aptasensor were obtained.The histamine content in four food samples were analyzed using the SERS aptasensor,with the results consistent with those determined by high performance liquid chromatography.The present work highlights the merits of indirect strategies for the ultrasensitive and highly selective SERS detection of small biological molecules in complex matrices.

关 键 词：Surface-enhanced Raman spectroscopy Raman biological-silent region APTAMER Histamine detection Universal SERS-tag 

分 类 号：O657.37[理学—分析化学] TS207.3[理学—化学]