脱落酸调控‘品丽珠’葡萄果实白藜芦醇合成的研究  

Study on Abscisic Acid Regulating Resveratrol Synthesis in‘Cabernet Franc’Grape

作　　者：王俊芳 姜凯凯 杨东岳 孙玉霞 管雪强 WANG Junfang;JIANG Kaikai;YANG Dongyue;SUN Yuxia;GUAN Xueqiang(Shandong Academy of Grape/Shandong Technology Innovation Center of Wine Grape and Wine,Jinan 250100,China)

机构地区：[1]山东省葡萄研究院/山东省酿酒葡萄与葡萄酒技术创新中心,山东济南250100

出　　处：《中外葡萄与葡萄酒》2024年第1期28-35,共8页Sino-Overseas Grapevine & Wine

基　　金：山东省自然科学基金面上项目(ZR2022MC168);山东省葡萄研究院引导基金(SDAG2021B01);国家自然科学基金青年基金(31701887);新疆生产建设兵团十二师重点领域科技攻关项目(SRS2022034);山东省现代农业产业技术体系项目(SDAIT-06-14);山东省科技型中小企业创新能力提升工程项目(2021TSGC1239)。

摘　　要：本研究在‘品丽珠’葡萄始熟期前10 d左右进行外源脱落酸(ABA)及其合成抑制剂氟啶酮(Fluridone)处理,以去离子水处理为对照,研究外源ABA对葡萄果实白藜芦醇(Resveratrol,Res)合成与积累的调控作用。结果发现,与对照相比,外源ABA处理能够促进‘品丽珠’葡萄果实提前成熟,采收时果实中可溶性固形物含量增加约5%,Res含量增加约33%;氟啶酮处理可以延缓葡萄果实成熟,采收时果实中可溶性固形物含量降低约20%,Res含量降低约44%;且葡萄果实发育过程中Res合成受转录水平的调控,外源ABA处理后ABA合成基因VvNCED1和VvNCED2表达升高,Res合成相关基因及其调控基因(VvSTS、VvMyb14及VvMyb15)表达升高,而氟啶酮处理均表现降低。进一步证明外源ABA有利于葡萄果实Res的合成和积累。Exogenous abscisic acid(ABA)and its synthesis inhibitor(fluridone)were applied on‘Cabernet Franc’(V.vinifera L.)at about 10 days before veraison and deionized water treatment was used as the control,to investigate the transcriptional regulatory mechanism of resveratrol(Res)synthesis.From the concentration of Res and gene expression analysis,ABA advanced berry ripening,and increased the total soluble solids(5%),and Res(33%)accumulation in berries;while fluridone delayed ripening,and decreased the total soluble solids(20%)and Res(44%)accumulation.However,the synthesis of Res was regulated on transcription level during berry ripening,two ABA synthesis genes(VvNCED1 and VvNCED2)were up-regulated,as well as Res synthesis genes and their regulatory genes(VvSTS,VvMyb 14 and VvMyb 15)were increased in exogenous ABA-applied berries.On the other hand,these genes were down-regulated in fluridone-applied berries.Therefore,this study can further verify exogenous ABA is beneficial to the synthesis and accumulation of Res in grape s.

关 键 词：白藜芦醇 脱落酸 品丽珠 基因表达 

分 类 号：S663.1[农业科学—果树学] S482.8[农业科学—园艺学]