ICP-MS法检测种植中药材中的无机溴化物含量  

Determination of the Content of Inorganic Bromide in Planted Traditional Chinese Medicine by ICP-MS

作　　者：武晓剑 姜悦 马艳宁 辛振强 殷红 钱勇 WU Xiaojian;JIANG Yue;MA Yanning;XIN Zhenqiang;YIN Hong;QIAN Yong(Shanghai Standard Technology Co.,Ltd.,Shanghai 201314,China;National Engineering Technology Research Center for TCM Modernization,TCM Standard Branch,Shanghai 201314,China;NingBo Lihua Pharmaceutical Co.,Ltd.,Ningbo 315174,China)

机构地区：[1]上海诗丹德标准技术服务有限公司,上海201314 [2]国家中药现代化工程技术研究中心中药标准分中心,上海201314 [3]宁波立华制药有限公司,浙江宁波315174

出　　处：《保鲜与加工》2024年第1期58-63,共6页Storage and Process

基　　金：上海市“科技创新行动计划”国内科技合作项目(20025800400)。

摘　　要：为建立电感耦合等离子体质谱仪(ICP-MS)检测人工种植中药中无机溴化物的分析方法,根据溴的特性确认ICP-MS测量质量数并考察了中药样品的提取方式、提取溶剂、提取时间对无机溴化物测试结果的影响。结果表明,中药样品中无机溴化物适宜的测试条件为:样品经粉碎后采用蒸馏水为提取溶剂超声提取溴化物,并用ICP-MS进行检测,内标法定量。此条件下无机溴化物质量浓度在0~0.20μg/mL范围内具有良好的线性(R2>0.999),加标回收率为109.9%~121.3%,相对标准偏差为1.8%(n=6),中间精密度为2.7%(n=12),方法检出限为1μg/g,定量限为2μg/g。该方法可用于中药无机溴化物的检测,其操作简单、灵敏度高。In order to establish an analytical method for the determination of inorganic bromide in artificially planted tra⁃ditional Chinese medicine(TCM)by inductively coupled plasma mass spectrometry(ICP-MS),the quantitative mass number of ICP-MS was confirmed based on the properties of bromine,and the effects of the extraction method,extrac⁃tion solvent and extraction time of inorganic bromide from TCM samples on the test results were investigated.The results showed that the suitable conditions for testing inorganic bromide in TCM samples were as follows:the inorganic bromide was extracted by ultrasound with distilled water as the extraction solvent after the samples were crushed,and then detect⁃ed by ICP-MS and quantified by internal standard method.The method showed good linearity in the range from 0 to 0.20μg/mL for inorganic bromide(R2>0.999),the spiked recoveries were 109.9%~121.3%with the relative standard devia⁃tions of 1.8%(n=6),the intermediate precision was 2.7%(n=12),the limits of detection and quantification were 1μg/g and 2μg/g,respectively.The method can be used for the detection of inorganic bromides in TCM with the simple opera⁃tion,and high sensitivity.

关 键 词：中药 无机溴化物 电感耦合等离子体质谱仪 检测 

分 类 号：TS207.53[轻工技术与工程—食品科学]