痰湿体质人群外周血淋巴细胞亚群及CD8^(+)T细胞受体免疫组库特征谱研究  

Characteristic Spectrum of Peripheral Lymphocyte Subsets and CD8^(+)T Lymphocyte Receptor Repertoire in Population with Tanshi(痰湿)Constitution

作　　者：郭姗姗 周海琴[2] 黄燕春[2] 田媛 毛楠[3,4] 黄祖波 任思冲[1,3,4] GUO Shanshan;ZHOU Haiqin;HUANG Yanchun;TIAN Yuan;MAO Nan;HUANG Zubo;REN Sichong(Sichuan Academy of Traditional Chinese Medicine/Center for TCM Translation Medicine of Sichuan Province,Chengdu 610041;The First People's Hospital of Longquanyi District of Chengdu/West China Hospital(Longquan)of Sichuan University,Chengdu 610100;The First Afiliated Hospital of Chengdu Medical College,Chengdu 610500;Sichuan Famous Traditional Chinese Medicine Doctor Fan Junming's Studio,Chengdu 610500;Sichuan Integrative Medicine Hospital,Chengdu1610042)

机构地区：[1]四川省中医药科学院/四川省中医药转化医学中心,成都610041 [2]成都市龙泉驿区第一人民医院/四川大学华西医院龙泉医院,成都610100 [3]成都医学院第一附属医院,成都610500 [4]四川省名中医樊均明工作室,成都610500 [5]四川省中西医结合医院,成都610042

出　　处：《中药药理与临床》2023年第11期82-86,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金青年基金项目(编号:81803967);四川中医药大健康产业发展与乡村振兴研究中心项目(编号:DJKYB202201、DJKZD202203);四川省科技计划重点研发项目(编号:2021YFS0035);四川省中医药管理局科研项目(编号:2020JC0024);四川省省级科研院所基本科研业务专项(A-2020N-25)。

摘　　要：目的:研究痰湿体质人群外周血淋巴细胞亚群分布和T细胞受体(TCR)免疫组库特征谱,以揭示痰湿体质机体免疫学特征。方法:流式细胞术检测平和体质和痰湿体质志愿者外周血淋巴细胞亚群分布,利用高通量测序技术对CD8^(+)T细胞受体β链(CD8^(+)TCRβ)基因测序,进行免疫组库分析。结果:与平和体质组比较,痰湿体质人群外周血中B淋巴细胞比率下降(P<0.05),而CD8^(+)T细胞和NKT细胞比率明显升高(P<0.05);痰湿体质组CD8^(+)TCRβ链存在2种V基因(TRBV7-4、TRBV10-2)、4种V-J组合基因(TRBV7-4_TRBJ2-7、TRBV7-2_TRBJ2-1、TRBV5-8_TRBJ2-1、TRBV7-8_TRBJ2-6)、5种V-D-J组合基因(TRBV6-5_TRBJ2-1_TRBD2、TRBV7-2_TRBJ2-5_TRBD2、TRBV4-1_TRBJ1-5_TRBD2、TRBV7-4_TRBJ2-5_TRBD2、TRBV6-8_TRBJ2-1_TRBD2)表达显著上调,2种V-J组合基因(TRBV27_TRBJ2-4和TRBV30_TRBJ2-2)及1种V-D-J组合基因(TRBV25-1_TRBJ2-5_TRBD1)表达显著下调。结论:痰湿体质外周血中淋巴细胞亚群分布和CD8^(+)T细胞受体表达谱具有体质特异性,本研究为进一步揭示痰湿体质人群免疫学特征提供了免疫组库方面的科学依据,同时为痰湿体质分子诊断及该体质易患疾病的发病机制研究提供新的研究思路。Objective:To study the distribution of peripheral lymphocyte subsets and the characteristic spectrum of T-cell receptor(TCR)immune repertoire in population with Tanshi(痰湿)constitution to uncover the immunological features of Tanshi constitution.Methods:Flow cytometry was used to analyze the distribution of peripheral lymphocyte subsets in volunteers with both the Pinghe(平和)constitution and the Tanshi constitution.High-throughput sequencing technology was employed to sequence the CD8^(+)TCRβchain(CD8^(+)TCRβ)gene,and immune repertoire analysis was conducted.Results:Compared with the Pinghe constitution group,the population with Tanshi constitution exhibited a decreased ratio of B lymphocytes in peripheral blood(P<0.05),while the ratios of CD8^(+)T cells and NKT cells were significantly elevated(P<0.05).In the Tanshi constitution group,the expression of CD8^(+)TCRβchains showed significant upregulation for 2 V genes(TRBV7-4,TRBV10-2),4 V-J combinations(TRBV7-4_TRBJ2-7,TRBV7-2_TRBJ2-1,TRBV5-8_TRBJ2-1,TRBV7-8_TRBJ2-6),and 5 V-D-J combinations(TRBV6-5_TRBJ2-1_TRBD2,TRBV7-2_TRBJ2-5_TRBD2,TRBV4-1_TRBJ1-5_TRBD2,TRBV7-4_TRBJ2-5_TRBD2,TRBV6-8_TRBJ2-1_TRBD2).Additionally,the expression of 2 V-J combinations(TRBV27_TRBJ2-4 and TRBV30_TRBJ2-2)and 1 V-D-J combination(TRBV25-1_TRBJ2-5_TRBD1)was significantly downregulated.Conclusion:The distribution of peripheral lymphocyte subsets and the expression profile of CD8^(+)TCR in the population with Tanshi constitution display constitution-specific characteristics.This study provides immunological repertoire-based scientific evidence for further understanding the immunological features of the population with Tanshi constitution.Moreover,it offers new research directions for the molecular diagnosis of Tanshi constitution and the investigation of its susceptibility mechanisms to diseases.

关 键 词：痰湿体质 淋巴细胞 免疫组库 高通量测序 

分 类 号：R229[医药卫生—中医基础理论]